Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2001 Aug:Chapter 19:Unit 19.7.
doi: 10.1002/0471142735.im1907s43.

A mouse model for cytomegalovirus infection

W Brune  1 , H Hengel, U H Koszinowski
Affiliations

A mouse model for cytomegalovirus infection

W Brune et al. Curr Protoc Immunol. 2001 Aug.

Abstract

This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles