Analysis of protein composition using multidimensional chromatography and mass spectrometry

doi:10.1002/0471140864.ps2301s34

. 2004 Feb:Chapter 23:23.1.1-23.1.25.

doi: 10.1002/0471140864.ps2301s34.

Analysis of protein composition using multidimensional chromatography and mass spectrometry

Andrew J Link¹, Jennifer L Jennings¹, Michael P Washburn²

Affiliations

PMID: 18429262
DOI: 10.1002/0471140864.ps2301s34

Analysis of protein composition using multidimensional chromatography and mass spectrometry

Andrew J Link et al. Curr Protoc Protein Sci. 2004 Feb.

. 2004 Feb:Chapter 23:23.1.1-23.1.25.

doi: 10.1002/0471140864.ps2301s34.

Authors

Andrew J Link¹, Jennifer L Jennings¹, Michael P Washburn²

Affiliations

¹ Vanderbilt University School of Medicine, Nashville, Tennessee.
² Torrey Mesa Research Institute, San Diego, California.

PMID: 18429262
DOI: 10.1002/0471140864.ps2301s34

Abstract

Multidimensional liquid chromatography of peptides produced by protease digestion of complex protein mixtures followed by tandem mass spectrometry can be coupled with automated database searching to identify large numbers of proteins in complex samples. These methods avoid the limitations of gel electrophoresis and in-gel digestions by directly identifying protein mixtures in solution.

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References

Literature Cited

References

1. Cao, P. and Stults, J.T. 2000. Mapping the phosphorylation sites of proteins using on-line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization multiple stage tandem mass spectrometry. Rapid Commun. Mass. Spectrom. 14:1600-1606.
1. Cortes, H.J., Pfeiffer, C.D., Richter, B.E., and Stevens, T.C. 1987. Porous ceramic bed supports for fused silica packed capillary columns used in liquid chromatography. J. High Res. Chrom. 10:446-448.
1. Davis, M.T., Beierle, J., Bures, E.T., McGinley, M.D., Mort, J., Robinson, J.H., Spahr, C.S., Yu, W., Luethy, R., and Patterson, S.D. 2001. Automated LC-LC-MS-MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses. J. Chromatogr. B. Biomed. Appl. 752:281-291.
1. Ficarro, S.B., McCleland, M.L., Stukenberg, P.T., Burke, D.J., Ross, M.M., Shabanowitz, J., Hunt, D.F., and White, F.M. 2002. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat. Biotechnol. 20:301-305.
1. Geng, M., Ji, J., and Regnier, F.E. 2000. Signature-peptide approach to detecting proteins in complex mixtures. J. Chromatogr. A 870:295-313.

Key References

1. Kinter, M. and Sherman, N.E. 2000. Protein Sequencing and Identification Using Tandem Mass Spectrometry. Wiley-Interscience, New York.
1. Link, et. al., 1999. See above.
1. Wolters, et. al., 2001. See above.

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[1] Cao, P. and Stults, J.T. 2000. Mapping the phosphorylation sites of proteins using on-line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization multiple stage tandem mass spectrometry. Rapid Commun. Mass. Spectrom. 14:1600-1606.

[2] Cao, P. and Stults, J.T. 2000. Mapping the phosphorylation sites of proteins using on-line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization multiple stage tandem mass spectrometry. Rapid Commun. Mass. Spectrom. 14:1600-1606.

[3] Cortes, H.J., Pfeiffer, C.D., Richter, B.E., and Stevens, T.C. 1987. Porous ceramic bed supports for fused silica packed capillary columns used in liquid chromatography. J. High Res. Chrom. 10:446-448.

[4] Cortes, H.J., Pfeiffer, C.D., Richter, B.E., and Stevens, T.C. 1987. Porous ceramic bed supports for fused silica packed capillary columns used in liquid chromatography. J. High Res. Chrom. 10:446-448.

[5] Davis, M.T., Beierle, J., Bures, E.T., McGinley, M.D., Mort, J., Robinson, J.H., Spahr, C.S., Yu, W., Luethy, R., and Patterson, S.D. 2001. Automated LC-LC-MS-MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses. J. Chromatogr. B. Biomed. Appl. 752:281-291.

[6] Davis, M.T., Beierle, J., Bures, E.T., McGinley, M.D., Mort, J., Robinson, J.H., Spahr, C.S., Yu, W., Luethy, R., and Patterson, S.D. 2001. Automated LC-LC-MS-MS platform using binary ion-exchange and gradient reversed-phase chromatography for improved proteomic analyses. J. Chromatogr. B. Biomed. Appl. 752:281-291.

[7] Ficarro, S.B., McCleland, M.L., Stukenberg, P.T., Burke, D.J., Ross, M.M., Shabanowitz, J., Hunt, D.F., and White, F.M. 2002. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat. Biotechnol. 20:301-305.

[8] Ficarro, S.B., McCleland, M.L., Stukenberg, P.T., Burke, D.J., Ross, M.M., Shabanowitz, J., Hunt, D.F., and White, F.M. 2002. Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat. Biotechnol. 20:301-305.

[9] Geng, M., Ji, J., and Regnier, F.E. 2000. Signature-peptide approach to detecting proteins in complex mixtures. J. Chromatogr. A 870:295-313.

[10] Geng, M., Ji, J., and Regnier, F.E. 2000. Signature-peptide approach to detecting proteins in complex mixtures. J. Chromatogr. A 870:295-313.

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Analysis of protein composition using multidimensional chromatography and mass spectrometry

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Analysis of protein composition using multidimensional chromatography and mass spectrometry

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