Coexpression of the mu-opioid receptor splice variant MOR1C and the vesicular glutamate transporter 2 (VGLUT2) in rat central nervous system
- PMID: 18381590
- DOI: 10.1002/cne.21712
Coexpression of the mu-opioid receptor splice variant MOR1C and the vesicular glutamate transporter 2 (VGLUT2) in rat central nervous system
Abstract
It has been reported that mu-opioid agonists depress glutamate release in some neurons but the specific receptor subtype mediating this effect is unclear. The purpose of the present study was to examine whether a particular mu-opioid receptor (MOR) splice-variant, MOR(1C), is expressed in rat central nervous system (CNS) by terminals expressing the vesicular glutamate transporter2 (VGLUT2), a marker of glutamatergic neurons. Several MOR splice variants have been identified in mice and MOR(1C) appears mainly to be localized to fibers and terminals, from which most neurotransmitter release would be expected. In addition, VGLUT2 has been found in the CNS and antibodies to it are reliable markers for glutamatergic terminals. Using fluorescence immunohistochemistry and confocal microscopy to examine spatial relationships between MOR(1C) and VGLUT2, we found that MOR(1C) and VGLUT2 puncta were widely distributed throughout the rat CNS; moreover, many regions contained terminals that expressed both. Thus, it appears that coexpression of MOR(1C) and VGLUT2 is common in the rat CNS. We hypothesize that activation of MOR(1C) by mu-opioid agonists at some glutamatergic terminals may be a mechanism by which glutamate release is inhibited.
Similar articles
-
Vesicular glutamate transporters, VGluT1 and VGluT2, in the trigeminal ganglion neurons of the rat, with special reference to coexpression.J Comp Neurol. 2003 Aug 18;463(2):212-20. doi: 10.1002/cne.10755. J Comp Neurol. 2003. PMID: 12815758
-
Axon terminals expressing vesicular glutamate transporter VGLUT1 or VGLUT2 within the trigeminal motor nucleus of the rat: origins and distribution patterns.J Comp Neurol. 2009 Feb 10;512(5):595-612. doi: 10.1002/cne.21894. J Comp Neurol. 2009. PMID: 19058187
-
Neuronal types expressing mu- and delta-opioid receptor mRNA in the rat hippocampal formation.J Comp Neurol. 2004 Jan 26;469(1):107-18. doi: 10.1002/cne.10997. J Comp Neurol. 2004. PMID: 14689476
-
Mu opiate receptor subtypes.Med Sci Monit. 2004 Jun;10(6):MS28-32. Epub 2004 Jun 1. Med Sci Monit. 2004. PMID: 15173681 Review.
-
Novel aspects of glutamatergic signalling in the neuroendocrine system.J Neuroendocrinol. 2008 Jun;20(6):743-51. doi: 10.1111/j.1365-2826.2008.01719.x. J Neuroendocrinol. 2008. PMID: 18601697 Review.
Cited by
-
Differential content of vesicular glutamate transporters in subsets of vagal afferents projecting to the nucleus tractus solitarii in the rat.J Comp Neurol. 2014 Feb 15;522(3):642-53. doi: 10.1002/cne.23438. J Comp Neurol. 2014. PMID: 23897509 Free PMC article.
-
Evidence for a role of glutamate as an efferent transmitter in taste buds.BMC Neurosci. 2010 Jun 21;11:77. doi: 10.1186/1471-2202-11-77. BMC Neurosci. 2010. PMID: 20565975 Free PMC article.
-
Effects of μ-opioid receptor modulation on the hippocampal network activity of sharp wave and ripples.Br J Pharmacol. 2013 Mar;168(5):1146-64. doi: 10.1111/j.1476-5381.2012.02240.x. Br J Pharmacol. 2013. PMID: 23043226 Free PMC article.
-
Differential localization of vesicular glutamate transporters and peptides in corneal afferents to trigeminal nucleus caudalis.J Comp Neurol. 2010 Sep 1;518(17):3557-69. doi: 10.1002/cne.22414. J Comp Neurol. 2010. PMID: 20593358 Free PMC article.
-
Hippocampal µ-opioid receptors on GABAergic neurons mediate stress-induced impairment of memory retrieval.Mol Psychiatry. 2020 May;25(5):977-992. doi: 10.1038/s41380-019-0435-z. Epub 2019 May 29. Mol Psychiatry. 2020. PMID: 31142818 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous
