Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C
- PMID: 18358815
- DOI: 10.1016/j.cell.2008.01.042
Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C
Abstract
Of all cells, human erythrocytes express the highest level of the Glut1 glucose transporter. However, the regulation and function of Glut1 during erythropoiesis are not known. Here, we report that glucose transport actually decreases during human erythropoiesis despite a >3-log increase in Glut1 transcripts. In contrast, Glut1-mediated transport of L-dehydroascorbic acid (DHA), an oxidized form of ascorbic acid (AA), is dramatically enhanced. We identified stomatin, an integral erythrocyte membrane protein, as regulating the switch from glucose to DHA transport. Notably though, we found that erythrocyte Glut1 and associated DHA uptake are unique traits of humans and the few other mammals that have lost the ability to synthesize AA from glucose. Accordingly, we show that mice, a species capable of synthesizing AA, express Glut4 but not Glut1 in mature erythrocytes. Thus, erythrocyte-specific coexpression of Glut1 with stomatin constitutes a compensatory mechanism in mammals that are unable to synthesize vitamin C.
Comment in
-
Some vertebrates go with the GLO.Cell. 2008 Mar 21;132(6):921-2. doi: 10.1016/j.cell.2008.03.005. Cell. 2008. PMID: 18358804
-
Altered GLUT1 substrate selectivity in human erythropoiesis?Cell. 2009 Apr 17;137(2):200-1; author reply 201-2. doi: 10.1016/j.cell.2009.04.007. Cell. 2009. PMID: 19379682 No abstract available.
Similar articles
-
Erythroid glucose transporters.Curr Opin Hematol. 2009 May;16(3):165-72. doi: 10.1097/MOH.0b013e328329905c. Curr Opin Hematol. 2009. PMID: 19346941 Review.
-
Some vertebrates go with the GLO.Cell. 2008 Mar 21;132(6):921-2. doi: 10.1016/j.cell.2008.03.005. Cell. 2008. PMID: 18358804
-
The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis.Blood. 2008 Dec 1;112(12):4729-38. doi: 10.1182/blood-2008-05-159269. Epub 2008 Sep 16. Blood. 2008. PMID: 18796630
-
Human erythrocytes transport dehydroascorbic acid and sugars using the same transporter complex.Am J Physiol Cell Physiol. 2014 May 15;306(10):C910-7. doi: 10.1152/ajpcell.00044.2014. Epub 2014 Mar 5. Am J Physiol Cell Physiol. 2014. PMID: 24598365 Free PMC article.
-
Vitamin C transporters.J Physiol Biochem. 2008 Dec;64(4):357-75. doi: 10.1007/BF03174092. J Physiol Biochem. 2008. PMID: 19391462 Review.
Cited by
-
Vitamin C Inhibits Blood-Stage Plasmodium Parasites via Oxidative Stress.Front Cell Dev Biol. 2021 May 11;9:639944. doi: 10.3389/fcell.2021.639944. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34046404 Free PMC article.
-
Vitamin C in mouse and human red blood cells: an HPLC assay.Anal Biochem. 2012 Jul 15;426(2):109-17. doi: 10.1016/j.ab.2012.04.014. Epub 2012 Apr 20. Anal Biochem. 2012. PMID: 22522059 Free PMC article.
-
Cation-leak stomatocytosis in standard schnauzers does not cosegregate with coding mutations in the RhAG, SLC4A1, or GLUT1 genes associated with human disease.Blood Cells Mol Dis. 2012 Apr 15;48(4):219-25. doi: 10.1016/j.bcmd.2012.02.003. Epub 2012 Mar 9. Blood Cells Mol Dis. 2012. PMID: 22406315 Free PMC article.
-
Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes.J Biol Chem. 2009 Oct 16;284(42):29218-29. doi: 10.1074/jbc.M109.014993. Epub 2009 Aug 20. J Biol Chem. 2009. PMID: 19696025 Free PMC article.
-
Identification of Copper Transporter 1 as a Receptor for Feline Endogenous Retrovirus ERV-DC14.J Virol. 2022 Jun 22;96(12):e0022922. doi: 10.1128/jvi.00229-22. Epub 2022 Jun 2. J Virol. 2022. PMID: 35652657 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous
