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. 2008 Sep;13(3):327-35.
doi: 10.1007/s12192-008-0031-7. Epub 2008 Mar 19.

Expression and localization of Hsps in the heart and blood vessel of heat-stressed broilers

Jimian Yu  1 , Endong Bao, Jianyan Yan, Lei Lei
Affiliations

Expression and localization of Hsps in the heart and blood vessel of heat-stressed broilers

Jimian Yu et al. Cell Stress Chaperones. 2008 Sep.

Abstract

The objective of this study was to investigate the kinetics of Hsp60, Hsp70, Hsp90 protein, and messenger RNA (mRNA) expression levels and to correlate these heat shock protein (Hsp) levels with tissue damage resulting from exposure to high temperatures for varying amounts of time. One hundred broilers were heat-stressed for 0, 2, 3, 5, and 10 h, respectively, by rapidly increasing the ambient temperature from 22 +/- 1 degrees C to 37 +/- 1 degrees C. Obvious elevations of plasma creatine kinase indicate damage to myocardial cells after heat stress. Hsp70 and Hsp90, and their corresponding mRNAs in the heart tissue of heat-stressed broilers, elevated significantly after 2 h of heat exposure and decreased quickly with continued heat stress. However, the levels of hsp60 mRNA in the heart of heat-stressed broilers increased sharply (P < 0.01) at 2 h of heat stress but then decreased quickly after 3 h, while the level of Hsp60 protein in the heart increased (P < 0.01) at 2 h of heat stress and maintained a high level throughout heat exposure. The results indicate that the elevation of the three Hsps, especially Hsp60 in heart, may be important markers at the beginning of heat stress and act as protective proteins in adverse environments. The reduction of Hsp signals in the cytoplasm of myocardial cells implies that myocardial cell lesions may have an adverse impact on the function of Hsps during heat stress. Meanwhile, the localization of Hsp70 in blood vessels of broiler hearts suggests another possible mechanism for protection of the heart after heat exposure.

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Figures

Fig. 1
Fig. 1
Levels of Hsp60 and hsp60 mRNA in the heart of heat-stressed broilers. *P < 0.05, **P < 0.01 when comparing the heat-stressed groups with the control. Values indicated are mean ± SD
Fig. 2
Fig. 2
Levels of Hsp70 and hsp70 mRNA in the heart of heat-stressed broilers. *P < 0.05, **P < 0.01 when comparing the heat stressed groups with the control. Values indicated are mean ± SD
Fig. 3
Fig. 3
Levels of Hsp90 and hsp90 mRNA in the heart of heat-stressed broilers. *P < 0.05, **P < 0.01 when comparing the heat stressed groups with the control. Values indicated are mean ± SD
Fig. 4
Fig. 4
Localization of Hsps in the myocardial cells of heat-stressed broilers (all images are at ×400 magnification). All images displayed the heart tissues of 3-h heat-stressed broilers. a Light pink granulation and loss of striations (right arrow) in the cytoplasm of the myocardial cells of heat-stressed broilers (HE staining); b Hsp60-positive signals were mainly detected in the cytoplasm of the myocardial cells (IHC staining), and Hsp60 staining was distinctly lower in the cytoplasm of granular degenerated areas (left arrow); c Strong positive signals of Hsp90 were detected both in the nucleus and the cytoplasm of the myocardial cells, mainly in the nucleus of the myocardial cells (IHC staining); d Positive staining of Hsp70 distributed both in the cytoplasm and the nucleus (IHC staining); e and f In the cross-section of the heart, positive signals of Hsp70 were localized in the endothelial cells (right arrow) and the wall of heart blood vessels (left arrow) of the heat-stressed broilers (IHC staining)
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