Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor
- PMID: 1833071
- DOI: 10.1016/0092-8674(91)90188-5
Adenovirus E1A activation domain binds the basic repeat in the TATA box transcription factor
Abstract
The adenovirus large E1A protein is a potent activator of transcription. We use several different experimental approaches to demonstrate that the large E1A protein binds specifically and stably to the TATA box-binding factor (TFIID), the general polymerase II transcription factor that initiates assembly of transcription complexes. Sedimentation velocity centrifugation revealed that TFIID and E1A form a heterodimer in vitro. We demonstrate that the activation domain of E1A (conserved region 3) binds to TFIID. E1A interacts with a 51 residue region from the conserved C-terminal domain of TFIID that includes a repeat of basic residues between the homologous direct repeats of TFIID. Analysis of TFIID binding by various E1A mutants indicates that TFIID binding is necessary, although not sufficient, for E1A transactivation.
Similar articles
-
Direct interaction between adenovirus E1A protein and the TATA box binding transcription factor IID.Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5124-8. doi: 10.1073/pnas.88.12.5124. Proc Natl Acad Sci U S A. 1991. PMID: 1828892 Free PMC article.
-
Functional interaction of adenovirus E1A with holo-TFIID.Genes Dev. 1993 Sep;7(9):1810-23. doi: 10.1101/gad.7.9.1810. Genes Dev. 1993. PMID: 8370528
-
Subregions of the adenovirus E1A transactivation domain target multiple components of the TFIID complex.Mol Cell Biol. 1995 Nov;15(11):6283-90. doi: 10.1128/MCB.15.11.6283. Mol Cell Biol. 1995. PMID: 7565781 Free PMC article.
-
Mechanisms of viral activators.Cold Spring Harb Symp Quant Biol. 1998;63:243-52. doi: 10.1101/sqb.1998.63.243. Cold Spring Harb Symp Quant Biol. 1998. PMID: 10384288 Review.
-
Retinoid-dependent transcription: the RAR/RXR-TBP-EIA/EIA-LA connection.Biochem Soc Symp. 1996;62:97-109. Biochem Soc Symp. 1996. PMID: 8971343 Review.
Cited by
-
A DNA element that regulates expression of an endogenous retrovirus during F9 cell differentiation is E1A dependent.Mol Cell Biol. 1992 Nov;12(11):4824-33. doi: 10.1128/mcb.12.11.4824-4833.1992. Mol Cell Biol. 1992. PMID: 1406664 Free PMC article.
-
Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau).Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11809-13. doi: 10.1073/pnas.89.24.11809. Proc Natl Acad Sci U S A. 1992. PMID: 1465404 Free PMC article.
-
Testifying the rice bacterial blight resistance gene xa5 by genetic complementation and further analyzing xa5 (Xa5) in comparison with its homolog TFIIAgamma1.Mol Genet Genomics. 2006 Apr;275(4):354-66. doi: 10.1007/s00438-005-0091-7. Epub 2006 Jan 28. Mol Genet Genomics. 2006. PMID: 16614777
-
The MyoD-inducible p204 protein overcomes the inhibition of myoblast differentiation by Id proteins.Mol Cell Biol. 2002 May;22(9):2893-905. doi: 10.1128/MCB.22.9.2893-2905.2002. Mol Cell Biol. 2002. PMID: 11940648 Free PMC article.
-
Transcriptional activators differ in their responses to overexpression of TATA-box-binding protein.Mol Cell Biol. 1995 Mar;15(3):1554-63. doi: 10.1128/MCB.15.3.1554. Mol Cell Biol. 1995. PMID: 7862148 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
