Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development
- PMID: 18295582
- DOI: 10.1016/j.cell.2008.02.008
Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development
Abstract
The potential to generate virtually any differentiated cell type from embryonic stem cells (ESCs) offers the possibility to establish new models of mammalian development and to create new sources of cells for regenerative medicine. To realize this potential, it is essential to be able to control ESC differentiation and to direct the development of these cells along specific pathways. Embryology has offered important insights into key pathways regulating ESC differentiation, resulting in advances in modeling gastrulation in culture and in the efficient induction of endoderm, mesoderm, and ectoderm and many of their downstream derivatives. This has led to the identification of new multipotential progenitors for the hematopoietic, neural, and cardiovascular lineages and to the development of protocols for the efficient generation of a broad spectrum of cell types including hematopoietic cells, cardiomyocytes, oligodendrocytes, dopamine neurons, and immature pancreatic beta cells. The next challenge will be to demonstrate the functional utility of these cells, both in vitro and in preclinical models of human disease.
Similar articles
-
Directed differentiation of pluripotent stem cells: from developmental biology to therapeutic applications.Cold Spring Harb Symp Quant Biol. 2008;73:101-10. doi: 10.1101/sqb.2008.73.065. Epub 2009 Mar 27. Cold Spring Harb Symp Quant Biol. 2008. PMID: 19329573 Review.
-
Propensity of human embryonic stem cell lines during early stage of lineage specification controls their terminal differentiation into mature cell types.Exp Biol Med (Maywood). 2009 Oct;234(10):1230-43. doi: 10.3181/0901-RM-38. Epub 2009 Jun 22. Exp Biol Med (Maywood). 2009. PMID: 19546356
-
Successful generation of donor specific hematopoietic stem cell lines from co-cultured bone marrow with human embryonic stem cell line: a new methodology.Transplant Proc. 2007 Apr;39(3):658-61. doi: 10.1016/j.transproceed.2007.01.048. Transplant Proc. 2007. PMID: 17445567
-
Neural commitment of embryonic stem cells: molecules, pathways and potential for cell therapy.J Pathol. 2008 Aug;215(4):355-68. doi: 10.1002/path.2380. J Pathol. 2008. PMID: 18566959 Review.
-
Derivation and characterization of canine embryonic stem cell lines with in vitro and in vivo differentiation potential.Stem Cells. 2009 Feb;27(2):329-40. doi: 10.1634/stemcells.2008-0433. Stem Cells. 2009. PMID: 19038794
Cited by
-
Derivation and 97% Purification of Human Thyroid Cells From Dermal Fibroblasts.Front Endocrinol (Lausanne). 2020 Jul 15;11:446. doi: 10.3389/fendo.2020.00446. eCollection 2020. Front Endocrinol (Lausanne). 2020. PMID: 32760346 Free PMC article.
-
X-chromosome inactivation in rett syndrome human induced pluripotent stem cells.Front Psychiatry. 2012 Mar 23;3:24. doi: 10.3389/fpsyt.2012.00024. eCollection 2012. Front Psychiatry. 2012. PMID: 22470355 Free PMC article.
-
Concise review: the relevance of human stem cell-derived organoid models for epithelial translational medicine.Stem Cells. 2013 Mar;31(3):417-22. doi: 10.1002/stem.1290. Stem Cells. 2013. PMID: 23203919 Free PMC article. Review.
-
DLX5 and HOXC8 enhance the chondrogenic differentiation potential of stem cells from apical papilla via LINC01013.Stem Cell Res Ther. 2020 Jul 6;11(1):271. doi: 10.1186/s13287-020-01791-8. Stem Cell Res Ther. 2020. PMID: 32631410 Free PMC article.
-
A review of human pluripotent stem cell-derived cardiomyocytes for high-throughput drug discovery, cardiotoxicity screening, and publication standards.J Cardiovasc Transl Res. 2013 Feb;6(1):22-30. doi: 10.1007/s12265-012-9423-2. Epub 2012 Nov 15. J Cardiovasc Transl Res. 2013. PMID: 23229562 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
