Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase
- PMID: 1825699
- DOI: 10.1038/349808a0
Fission yeast p107wee1 mitotic inhibitor is a tyrosine/serine kinase
Abstract
The fission yeast wee1+ gene product is a dose-dependent, negative regulator of entry into mitosis. wee1+ encodes a protein of relative molecular mass 107,000 (Mr 107K), the C-terminal third of which has strong similarities with the serine/threonine protein kinase family. Here we report that p107wee1 immune complexes phosphorylate p107wee1 equally on serine and tyrosine residues, and also phosphorylate an exogenous substrate, angiotensin II, on tyrosine. Both kinase activities are attributable to p107wee1 because they are also observed when wee1+ is expressed in heterologous systems; both are abolished by a point mutation in the ATP-binding domain, and both behave like an asymmetric monomer of Mr114K on gel filtration and density-gradient centrifugation. Thus the wee1+ gene product is representative of a novel class of protein kinase that phosphorylates both serine and tyrosine residues.
Similar articles
-
Phosphorylation and inactivation of the mitotic inhibitor Wee1 by the nim1/cdr1 kinase.Nature. 1993 Jun 24;363(6431):736-8. doi: 10.1038/363736a0. Nature. 1993. PMID: 8515817
-
Nim1 kinase promotes mitosis by inactivating Wee1 tyrosine kinase.Nature. 1993 Jun 24;363(6431):738-41. doi: 10.1038/363738a0. Nature. 1993. PMID: 8515818
-
Tyrosine phosphorylation of the fission yeast cdc2+ protein kinase regulates entry into mitosis.Nature. 1989 Nov 2;342(6245):39-45. doi: 10.1038/342039a0. Nature. 1989. PMID: 2682257
-
Radiation-induced mitotic delay: a genetic characterization in the fission yeast.Radiat Res. 1992 Nov;132(2):144-52. Radiat Res. 1992. PMID: 1438695 Review.
-
Genetic interactions in the control of mitosis in fission yeast.Curr Genet. 1989 Jul;16(1):1-6. doi: 10.1007/BF00411076. Curr Genet. 1989. PMID: 2571422 Review. No abstract available.
Cited by
-
Mutations of p34cdc2 phosphorylation sites induce premature mitotic events in HeLa cells: evidence for a double block to p34cdc2 kinase activation in vertebrates.EMBO J. 1991 Nov;10(11):3331-41. doi: 10.1002/j.1460-2075.1991.tb04897.x. EMBO J. 1991. PMID: 1655418 Free PMC article.
-
An extra copy of nimEcyclinB elevates pre-MPF levels and partially suppresses mutation of nimTcdc25 in Aspergillus nidulans.EMBO J. 1992 Jun;11(6):2139-49. doi: 10.1002/j.1460-2075.1992.tb05273.x. EMBO J. 1992. PMID: 1534750 Free PMC article.
-
Wee1 kinase alters cyclin E/Cdk2 and promotes apoptosis during the early embryonic development of Xenopus laevis.BMC Dev Biol. 2007 Oct 25;7:119. doi: 10.1186/1471-213X-7-119. BMC Dev Biol. 2007. PMID: 17961226 Free PMC article.
-
The existence of two distinct Wee1 isoforms in Xenopus: implications for the developmental regulation of the cell cycle.EMBO J. 2002 May 15;21(10):2472-84. doi: 10.1093/emboj/21.10.2472. EMBO J. 2002. PMID: 12006499 Free PMC article.
-
The Xenopus Chk1 protein kinase mediates a caffeine-sensitive pathway of checkpoint control in cell-free extracts.J Cell Biol. 1998 Sep 21;142(6):1559-69. doi: 10.1083/jcb.142.6.1559. J Cell Biol. 1998. PMID: 9744884 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
