doi: 10.1016/j.bbrc.2007.12.189.
Epub 2008 Jan 14.
PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells
Affiliations
- PMID: 18194669
- DOI: 10.1016/j.bbrc.2007.12.189
Item in Clipboard
PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells
Biochem Biophys Res Commun.
.
Abstract
PRDM14 was identified by microarray analysis and was expressed in specifically undifferentiated human ES cells. PRDM14 protein is thought to regulate gene transcription in human ES cells, as it contains a PR domain, a subtype of the SET domain which catalyzes histone methylation. To analyze the function of PRDM14, we performed knock-down and forced expression of PRDM14 in human ES cells. Knock-down of PRDM14 by siRNA induced expression of early differentiation marker genes. Forced expression of PRDM14 suppressed expression of differentiation marker genes in the embryoid body. These results suggest that PRDM14 is involved in the maintenance of the self-renewal of human ES cells by suppression of gene expression.
Similar articles
-
Small increases in the level of Sox2 trigger the differentiation of mouse embryonic stem cells.Stem Cells. 2008 Apr;26(4):903-11. doi: 10.1634/stemcells.2007-0951. Epub 2008 Jan 31. Stem Cells. 2008. PMID: 18238855
-
Molecular analysis of LEFTY-expressing cells in early human embryoid bodies.Stem Cells. 2007 Feb;25(2):465-72. doi: 10.1634/stemcells.2006-0179. Epub 2006 Oct 12. Stem Cells. 2007. PMID: 17038673
-
Hoxa1 is required for the retinoic acid-induced differentiation of embryonic stem cells into neurons.J Neurosci Res. 2008 Oct;86(13):2809-19. doi: 10.1002/jnr.21729. J Neurosci Res. 2008. PMID: 18512762
-
Functions of the TGFbeta superfamily in human embryonic stem cells.APMIS. 2005 Nov-Dec;113(11-12):773-89. doi: 10.1111/j.1600-0463.2005.apm_3181.x. APMIS. 2005. PMID: 16480449 Review.
-
Transcriptional repression in ES cells.J Cell Biochem. 2010 May 15;110(2):288-93. doi: 10.1002/jcb.22576. J Cell Biochem. 2010. PMID: 20336663 Review.
Cited by
-
PRDM14 is overexpressed in chronic pancreatitis prior to pancreatic cancer.FEBS Open Bio. 2018 Sep 17;8(10):1733-1741. doi: 10.1002/2211-5463.12519. eCollection 2018 Oct. FEBS Open Bio. 2018. PMID: 30338223 Free PMC article.
-
Exploring induced pluripotency in human fibroblasts via construction, validation, and application of a gene regulatory network.PLoS One. 2019 Aug 2;14(8):e0220742. doi: 10.1371/journal.pone.0220742. eCollection 2019. PLoS One. 2019. PMID: 31374103 Free PMC article.
-
Therapeutic siRNA targeting the cancer cell stemness regulator PRDI-BF1 and RIZ domain zinc finger protein 14.Proc Jpn Acad Ser B Phys Biol Sci. 2022;98(7):325-335. doi: 10.2183/pjab.98.017. Proc Jpn Acad Ser B Phys Biol Sci. 2022. PMID: 35908955 Free PMC article.
-
YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5. Dev Cell. 2022. PMID: 34990589 Free PMC article.
-
The transcriptional and signalling networks of pluripotency.Nat Cell Biol. 2011 May;13(5):490-6. doi: 10.1038/ncb0511-490. Nat Cell Biol. 2011. PMID: 21540844 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
