A major EBNA1 variant from Asian EBV isolates shows enhanced transcriptional activity compared to prototype B95.8
- PMID: 18096263
- DOI: 10.1016/j.virusres.2007.10.020
A major EBNA1 variant from Asian EBV isolates shows enhanced transcriptional activity compared to prototype B95.8
Abstract
Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) has an instrumental role in maintaining EBV latent infection by controlling EBV episome replication and regulating viral transcription. It is a ubiquitously expressed protein during latent viral infection and in EBV-associated tumors. The EBNA1 C-terminus interacts functionally with the Qp and Cp that control viral gene expression in latency I/II and III, respectively. EBNA1 has been classified into five subtypes due to sequence variation in the DNA-interacting C-terminus. By DNA sequence analysis of its C-terminus, we detected a main sub-variant (V-val-v1) of EBNA1 with valine located in both positions 487 and 528 from matched samples including NPC biopsies and peripheral blood taken from Vietnamese (9), Chinese (12) NPC patients and healthy donors (5). In the FR-region of oriP from nine NPC biopsies from Vietnam we also frequently found substitutions, deletions and variable numbers of repeats. Using a luciferase reporter system, EBNA1 and FR both derived from Asian isolates induced higher transcriptional activity than those from B95-8 virus.
Similar articles
-
Epstein-Barr virus genetic variation in Vietnamese patients with nasopharyngeal carcinoma: full-length analysis of LMP1.Virus Genes. 2008 Oct;37(2):273-81. doi: 10.1007/s11262-008-0262-9. Epub 2008 Jul 29. Virus Genes. 2008. PMID: 18663567
-
Structural Basis for Cooperative Binding of EBNA1 to the Epstein-Barr Virus Dyad Symmetry Minimal Origin of Replication.J Virol. 2019 Sep 30;93(20):e00487-19. doi: 10.1128/JVI.00487-19. Print 2019 Oct 15. J Virol. 2019. PMID: 31142669 Free PMC article.
-
Carcinoma-risk variant of EBNA1 deregulates Epstein-Barr Virus episomal latency.Oncotarget. 2017 Jan 31;8(5):7248-7264. doi: 10.18632/oncotarget.14540. Oncotarget. 2017. PMID: 28077791 Free PMC article.
-
Epstein-Barr virus-encoded EBNA1 and ZEBRA: targets for therapeutic strategies against EBV-carrying cancers.J Pathol. 2015 Jan;235(2):334-41. doi: 10.1002/path.4431. J Pathol. 2015. PMID: 25186125 Review.
-
EBNA1.Curr Top Microbiol Immunol. 2015;391:3-34. doi: 10.1007/978-3-319-22834-1_1. Curr Top Microbiol Immunol. 2015. PMID: 26428370 Review.
Cited by
-
Epstein-barr virus sequence variation-biology and disease.Pathogens. 2012 Nov 8;1(2):156-74. doi: 10.3390/pathogens1020156. Pathogens. 2012. PMID: 25436768 Free PMC article. Review.
-
Characterization of a novel wood mouse virus related to murid herpesvirus 4.J Gen Virol. 2010 Apr;91(Pt 4):867-79. doi: 10.1099/vir.0.017327-0. Epub 2009 Nov 25. J Gen Virol. 2010. PMID: 19940063 Free PMC article.
-
Genome-Wide Analysis of Epstein-Barr Virus Isolated from Extranodal NK/T-Cell Lymphoma, Nasal Type.Oncologist. 2019 Sep;24(9):e905-e913. doi: 10.1634/theoncologist.2017-0588. Epub 2019 Apr 2. Oncologist. 2019. PMID: 30940744 Free PMC article.
-
Sequence Variation Analysis of Epstein-Barr Virus Nuclear Antigen 1 Gene in the Virus Associated Lymphomas of Northern China.PLoS One. 2015 Oct 13;10(10):e0140529. doi: 10.1371/journal.pone.0140529. eCollection 2015. PLoS One. 2015. PMID: 26460969 Free PMC article.
-
EBV and the Pathogenesis of NK/T Cell Lymphoma.Cancers (Basel). 2021 Mar 19;13(6):1414. doi: 10.3390/cancers13061414. Cancers (Basel). 2021. PMID: 33808787 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
