Dengue virus infection induces passive release of high mobility group box 1 protein by epithelial cells
- PMID: 18076993
- DOI: 10.1016/j.jinf.2007.10.008
Dengue virus infection induces passive release of high mobility group box 1 protein by epithelial cells
Abstract
Objectives: Dengue hemorrhagic fever and dengue shock syndrome (DHF/DSS) are severe complications of secondary dengue virus (DV) infection. In the current study, we provide the first evidence of induction of cellular necrosis by DV type 2 (DV-2).
Methods and results: The epithelial cell line A549 can support replication of dengue virus as demonstrated by expression of viral NS1 antigen and virus plaque assay. DV-2 infection of cells induced cell death in approximately half of the cells that were actively infected. Using sodium 3'-[1-(phenylaminocarbonyl)-3, 4-tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate [XTT]-based cell viability assays, we found that DV-2 infection at a multiplicity of infection (MOI) of 10 resulted in significant death of cells as well as high extracellular lactate dehydrogenase (LDH) activity and leakage of the high mobility group 1 (HMGB1) protein into the extracellular space.
Conclusions: These results suggest that HMGB1 may be a signal of tissue or cellular injury by DV-2, which in turn is likely to induce and/or enhance an immune reaction.
Similar articles
-
Implications of the release of high-mobility group box 1 protein from dying cells during human immunodeficiency virus type 1 infection in vitro.J Gen Virol. 2010 Jul;91(Pt 7):1800-9. doi: 10.1099/vir.0.016915-0. Epub 2010 Mar 3. J Gen Virol. 2010. PMID: 20200191
-
CLEC5A is critical for dengue-virus-induced lethal disease.Nature. 2008 May 29;453(7195):672-6. doi: 10.1038/nature07013. Epub 2008 May 21. Nature. 2008. PMID: 18496526
-
Tissue plasminogen activator induced by dengue virus infection of human endothelial cells.J Med Virol. 2003 Aug;70(4):610-6. doi: 10.1002/jmv.10438. J Med Virol. 2003. PMID: 12794725
-
Induction of programmed cell death (apoptosis) by dengue virus in vitro and in vivo.Acta Cient Venez. 1998;49 Suppl 1:13-7. Acta Cient Venez. 1998. PMID: 10030049 Review.
-
Alternate hypothesis on the pathogenesis of dengue hemorrhagic fever (DHF)/dengue shock syndrome (DSS) in dengue virus infection.Exp Biol Med (Maywood). 2008 Apr;233(4):401-8. doi: 10.3181/0707-MR-198. Exp Biol Med (Maywood). 2008. PMID: 18367628 Review.
Cited by
-
Endothelial Dysfunction, HMGB1, and Dengue: An Enigma to Solve.Viruses. 2022 Aug 12;14(8):1765. doi: 10.3390/v14081765. Viruses. 2022. PMID: 36016387 Free PMC article. Review.
-
Pathogen-induced inflammation is attenuated by the iminosugar MON-DNJ via modulation of the unfolded protein response.Immunology. 2021 Nov;164(3):587-601. doi: 10.1111/imm.13393. Epub 2021 Aug 1. Immunology. 2021. PMID: 34287854 Free PMC article.
-
Influence of Herbal Medicines on HMGB1 Release, SARS-CoV-2 Viral Attachment, Acute Respiratory Failure, and Sepsis. A Literature Review.Int J Mol Sci. 2020 Jun 30;21(13):4639. doi: 10.3390/ijms21134639. Int J Mol Sci. 2020. PMID: 32629817 Free PMC article. Review.
-
Myeloid Cells during Viral Infections and Inflammation.Viruses. 2019 Feb 19;11(2):168. doi: 10.3390/v11020168. Viruses. 2019. PMID: 30791481 Free PMC article. Review.
-
Dissecting host cell death programs in the pathogenesis of influenza.Microbes Infect. 2018 Oct-Nov;20(9-10):560-569. doi: 10.1016/j.micinf.2018.03.005. Epub 2018 Apr 18. Microbes Infect. 2018. PMID: 29679740 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous