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Clinical Trial
. 2007 Dec 14;13(46):6269-73.
doi: 10.3748/wjg.v13.i46.6269.

Relationship between vascular invasion and microvessel density and micrometastasis

Yan-Dong Wang  1 , Pei Wu, Jia-Ding Mao, He Huang, Fan Zhang
Affiliations
Clinical Trial

Relationship between vascular invasion and microvessel density and micrometastasis

Yan-Dong Wang et al. World J Gastroenterol. .

Abstract

Aim: To evaluate the relationship between vascular invasion and microvessel density (MVD) of tissue and micrometastasis in blood.

Methods: Vascular invasion was detected by both hematoxylin and eosin staining and immunohistochemiscal staining. Blood samples were collected from 17 patients with vascular invasion and 29 patients without vascular invasion and examined for cytokeratin20 (CK20) expression by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Microvessel density of tissue samples was also determined by immunohistochemistry using antibodies to CD105.

Results: CK20 was detected in 12 of the 17 patients with vascular invasion and in 9 of the 29 patients without vascular invasion. Positive RT-PCR was significantly correlated with vascular invasion (70.6% vs 30.0%, P < 0.05). The average MVD was significantly higher in patients with positive vascular invasion than in patients with negative vascular invasion (29.2 +/- 3.3 vs 25.4 +/- 4.7, P < 0.05). The vascular invasion detected with hematoxylin-eosin staining was less than that with immunohistochemical staining. There was a significant difference between the two staining methods (19.6% vs 36.9%, P < 0.05).

Conclusion: Positive CK20 RT-PCR, depth of tumor invasion, lymph node status, metastasis and MVD are significantly correlated with vascular invasion. Immunohistochemical staining is more sensitive than hematoxylin-eosin staining for detecting vascular invasion.

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Figures

Figure 1
Figure 1
Immunohistochemical staining (A) and hematoxylin-eosin staining (B) of tumor cells (× 400) showing a tumor cell cluster in vascular spaces with brown-stained endothelial cells and tumor cells in blood vessel spaces with erythrocytes surrounded.
Figure 2
Figure 2
Expression of both CK20 mRNA and GAPDH detected in six patients and expression of only GAPDH detected in five patients.
See this image and copyright information in PMC

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References

    1. Minsky BD, Cohen AM. Blood vessel invasion in colorectal cancer--an alternative to TNM staging? Ann Surg Oncol. 1999;6:129–130. - PubMed
    1. Mullis KB. Target amplification for DNA analysis by the polymerase chain reaction. Ann Biol Clin (Paris) 1990;48:579–582. - PubMed
    1. Majima T, Ichikura T, Takayama E, Chochi K, Mochizuki H. Detecting circulating cancer cells using reverse transcriptase-polymerase chain reaction for cytokeratin mRNA in peripheral blood from patients with gastric cancer. Jpn J Clin Oncol. 2000;30:499–503. - PubMed
    1. Wildi S, Kleeff J, Maruyama H, Maurer CA, Friess H, Büchler MW, Lander AD, Korc M. Characterization of cytokeratin 20 expression in pancreatic and colorectal cancer. Clin Cancer Res. 1999;5:2840–2847. - PubMed
    1. McDonnell CO, Hill AD, McNamara DA, Walsh TN, Bouchier-Hayes DJ. Tumour micrometastases: the influence of angiogenesis. Eur J Surg Oncol. 2000;26:105–115. - PubMed

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