Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Oct;13(5):452-61.
doi: 10.1080/13550280701510104.

Host and virus strain dependence in activation of human macrophages by human immunodeficiency virus type 1

Katarzyna Kazmierczak  1 , Mary Jane Potash
Affiliations

Host and virus strain dependence in activation of human macrophages by human immunodeficiency virus type 1

Katarzyna Kazmierczak et al. J Neurovirol. 2007 Oct.

Abstract

Human immunodeficiency virus type 1 (HIV-1)-associated neuropathogenesis occurs in a large minority of infected people. Presently, there are neither viral nor cellular markers that predict the development of brain disease during HIV-1 infection. This study was conducted to determine whether there exist systematic differences among human cell donors and virus strains for the activation of macrophage gene expression by HIV-1 that may contribute to neuropathogenesis. Four HIV-1, ADA and B-aL, which were isolated from peripheral tissues of acquired immunodeficiency syndrome (AIDS) patients, and DJV and YU-2, which were isolated from brains of patients with HIV-1-associated dementia, were compared for induction of expression of cellular genes associated with antiviral activity or inflammation in monocyte-derived macrophages from several donors. Virus replication and cytokine production were scored by enzyme-linked immunosorbent assay (ELISA) and cellular transcripts were measured by real-time polymerase chain reaction (PCR). ADA and B-aL productively infected cells from all donors tested and induced all cellular transcripts tested, illustrating a common response of macrophages to HIV-1 replication. In sharp contrast, the viruses associated with neuropathogenesis, DJV and YU-2, induced intense gene expression early after infection in cells from a subset of donors but DJV did not productively infect these cells. No such heterogeneity was observed in the responses of macrophages during high-level replication of any HIV-1 tested. The susceptibility to early activation by HIV-1 may reflect susceptibility to neuropathogenesis in AIDS.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Virology. 2001 Jan 20;279(2):509-26 - PubMed
    1. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4832-7 - PubMed
    1. Ann Neurol. 1995 Oct;38(4):563-70 - PubMed
    1. J Infect Dis. 1998 Oct;178(4):1000-7 - PubMed
    1. J Virol. 1991 Aug;65(8):3973-85 - PubMed

Publication types

MeSH terms

LinkOut - more resources