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. 2007 Oct 3;129(39):11900-1.
doi: 10.1021/ja073936h. Epub 2007 Sep 12.

Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays

Jelena Levi  1 , Abhijit De, Zhen Cheng, Sanjiv Sam Gambhir
Affiliations

Bisdeoxycoelenterazine derivatives for improvement of bioluminescence resonance energy transfer assays

Jelena Levi et al. J Am Chem Soc. .
No abstract available

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Figures

Figure 1
Figure 1
Synthesized bisdeoxycoelenterazine derivatives: acetyl bis-deoxycoelenterazine (7), O-Boc-bisdeoxycoelenterazine (8), acetoxymethyl bisdeoxycoelenterazine (9), pivaloyloxymethyl bisdeoxycoelenterazine (10).
Figure 2
Figure 2
The rate of the bioluminescent reaction for BDC (◆), acetyl-BDC (■), O-Boc-BDC (●), acetoxymethyl-BDC (▲), and pivaloyloxy-methyl-BDC (□) evaluated as the change of the maximum light signal over time.
Figure 3
Figure 3
(a) The change in luminescence over time for acetyl bisdeoxy-coelenterazine (7), O-Boc-bisdeoxycoelenterazine (8), acetoxymethyl bis-deoxycoelenterazine (9), and pivaloyloxymethyl bisdeoxycoelenterazine (10). Error bars represent standard deviation from the average value. (b) Bioluminescence imaging of HT1080 cells expressing GFP-RLuc fusion protein after exposure to bisdeoxycoelenterazine and its derivatives. Color bar units are p/s/cm2/sr, where p is for photon and sr is for steradian.
Scheme 1
Scheme 1
Mechanism of Coelenterazine Oxidation and Light Production
See this image and copyright information in PMC

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