Wnt5a promotes adhesion of human dermal fibroblasts by triggering a phosphatidylinositol-3 kinase/Akt signal
- PMID: 17804197
- DOI: 10.1016/j.cellsig.2007.07.023
Wnt5a promotes adhesion of human dermal fibroblasts by triggering a phosphatidylinositol-3 kinase/Akt signal
Abstract
Frizzled-3 (Fzd3), highly expressed in both the central nervous system (CNS) and skin, plays essential roles in axonal growth and guidance during the CNS development and may be involved in maintenance of skin integrity, although its ligand remains undetermined. In this study, we demonstrate that Wnt5a specifically binds to Fzd3 in vitro and triggers phosphorylation of Akt mediated by phosphatidylinositol-3 kinase (PI3K), but not that of ERK or protein kinase C, in human primary-cultured dermal fibroblasts. We have further found that such Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal promotes integrin-mediated adhesion of human dermal fibroblasts to collagen I-coated dishes. Based on another finding that Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal was blocked by an excess amount of a recombinant Fzd3-cysteine-rich domain (CRD), but not by that of a recombinant Fzd6-CRD, it is concluded that Wnt5a is a natural ligand of Fzd3 that triggers the PI3K/Akt signal and promotes adhesion of human dermal fibroblasts.
Similar articles
-
Epidermal growth factor up-regulates expression of transforming growth factor beta receptor type II in human dermal fibroblasts by phosphoinositide 3-kinase/Akt signaling pathway: Resistance to epidermal growth factor stimulation in scleroderma fibroblasts.Arthritis Rheum. 2003 Jun;48(6):1652-66. doi: 10.1002/art.11029. Arthritis Rheum. 2003. PMID: 12794834
-
PI3K/Akt-dependent phosphorylation of GSK3β and activation of RhoA regulate Wnt5a-induced gastric cancer cell migration.Cell Signal. 2013 Feb;25(2):447-56. doi: 10.1016/j.cellsig.2012.10.012. Epub 2012 Oct 31. Cell Signal. 2013. PMID: 23123500
-
Regulation of interleukin-5-induced beta2-integrin adhesion of human eosinophils by phosphoinositide 3-kinase.Am J Respir Cell Mol Biol. 2005 Jul;33(1):65-70. doi: 10.1165/rcmb.2005-0076OC. Epub 2005 Mar 31. Am J Respir Cell Mol Biol. 2005. PMID: 15802551 Free PMC article.
-
An essential role of integrin-linked kinase in the cellular radiosensitivity of normal fibroblasts during the process of cell adhesion and spreading.Int J Radiat Biol. 2007 Nov-Dec;83(11-12):769-79. doi: 10.1080/09553000701694327. Int J Radiat Biol. 2007. PMID: 17952767
-
Upregulation of tenascin-C expression by IL-13 in human dermal fibroblasts via the phosphoinositide 3-kinase/Akt and the protein kinase C signaling pathways.J Invest Dermatol. 2006 Mar;126(3):551-60. doi: 10.1038/sj.jid.5700090. J Invest Dermatol. 2006. PMID: 16374482
Cited by
-
Arrestin development: emerging roles for beta-arrestins in developmental signaling pathways.Dev Cell. 2009 Oct;17(4):443-58. doi: 10.1016/j.devcel.2009.09.011. Dev Cell. 2009. PMID: 19853559 Free PMC article. Review.
-
Common dysregulation of Wnt/Frizzled receptor elements in human hepatocellular carcinoma.Br J Cancer. 2008 Jul 8;99(1):143-50. doi: 10.1038/sj.bjc.6604422. Epub 2008 Jun 24. Br J Cancer. 2008. PMID: 18577996 Free PMC article.
-
Expression of WNT5A in Idiopathic Pulmonary Fibrosis and Its Control by TGF-β and WNT7B in Human Lung Fibroblasts.J Histochem Cytochem. 2016 Feb;64(2):99-111. doi: 10.1369/0022155415617988. Epub 2015 Nov 4. J Histochem Cytochem. 2016. PMID: 26538547 Free PMC article.
-
Functional role of the Frizzled linker domain in the Wnt signaling pathway.Commun Biol. 2022 May 5;5(1):421. doi: 10.1038/s42003-022-03370-4. Commun Biol. 2022. PMID: 35513706 Free PMC article.
-
WNT-5A: signaling and functions in health and disease.Cell Mol Life Sci. 2016 Feb;73(3):567-87. doi: 10.1007/s00018-015-2076-y. Epub 2015 Oct 29. Cell Mol Life Sci. 2016. PMID: 26514730 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
