Cyclin-dependent kinases control septin phosphorylation in Candida albicans hyphal development
- PMID: 17765684
- DOI: 10.1016/j.devcel.2007.06.011
Cyclin-dependent kinases control septin phosphorylation in Candida albicans hyphal development
Abstract
Cyclin-dependent kinases (Cdks) control cytoskeleton polarization in yeast morphogenesis. However, the target and mechanism remain unclear. Here, we show that the Candida albicans Cdk Cdc28, through temporally controlled association with two cyclins Ccn1 and Hgc1, rapidly establishes and persistently maintains phosphorylation of the septin cytoskeleton protein Cdc11 for hyphal development. Upon hyphal induction, Cdc28-Ccn1 binds to septin complexes and phosphorylates Cdc11 on Ser394, a nonconsensus Cdk target. This phosphorylation requires prior phosphorylation on Ser395 by the septin-associated kinase Gin4. Mutating Ser394 or Ser395 blocked Cdc11 phosphorylation on Ser394 and impaired hyphal morphogenesis. Reconstitution experiments using purified Cdc28-Ccn1, Gin4, and septins reproduced phosphorylations on the same residues. Transient septin-Cdc28 associations were also detected prior to bud and mating-projection emergence in S. cerevisiae. Our study uncovers a direct link between the cell-cycle engine and the septin cytoskeleton that may be part of a conserved mechanism underlying polarized morphogenesis.
Comment in
-
Morphogenesis of a human fungal pathogen requires septin phosphorylation.Dev Cell. 2007 Sep;13(3):315-6. doi: 10.1016/j.devcel.2007.08.009. Dev Cell. 2007. PMID: 17765672
Similar articles
-
Morphogenesis of a human fungal pathogen requires septin phosphorylation.Dev Cell. 2007 Sep;13(3):315-6. doi: 10.1016/j.devcel.2007.08.009. Dev Cell. 2007. PMID: 17765672
-
Hgc1, a novel hypha-specific G1 cyclin-related protein regulates Candida albicans hyphal morphogenesis.EMBO J. 2004 Apr 21;23(8):1845-56. doi: 10.1038/sj.emboj.7600195. Epub 2004 Apr 8. EMBO J. 2004. PMID: 15071502 Free PMC article.
-
CDKs and the yeast-hyphal decision.Curr Opin Microbiol. 2009 Dec;12(6):644-9. doi: 10.1016/j.mib.2009.09.002. Curr Opin Microbiol. 2009. PMID: 19837628 Review.
-
Candida albicans hyphal morphogenesis occurs in Sec3p-independent and Sec3p-dependent phases separated by septin ring formation.J Cell Sci. 2007 Jun 1;120(Pt 11):1898-907. doi: 10.1242/jcs.002931. Epub 2007 May 15. J Cell Sci. 2007. PMID: 17504812
-
Hgc1-Cdc28-how much does a single protein kinase do in the regulation of hyphal development in Candida albicans?J Microbiol. 2016 Mar;54(3):170-7. doi: 10.1007/s12275-016-5550-9. Epub 2016 Feb 27. J Microbiol. 2016. PMID: 26920877 Review.
Cited by
-
Hgc1 Independence of Biofilm Hyphae in Candida albicans.mBio. 2023 Apr 25;14(2):e0349822. doi: 10.1128/mbio.03498-22. Epub 2023 Feb 13. mBio. 2023. PMID: 36779720 Free PMC article.
-
Candida albicans hyphal initiation and elongation.Trends Microbiol. 2014 Dec;22(12):707-14. doi: 10.1016/j.tim.2014.09.001. Epub 2014 Sep 25. Trends Microbiol. 2014. PMID: 25262420 Free PMC article. Review.
-
Septins and Generation of Asymmetries in Fungal Cells.Annu Rev Microbiol. 2015;69:487-503. doi: 10.1146/annurev-micro-091014-104250. Annu Rev Microbiol. 2015. PMID: 26488282 Free PMC article. Review.
-
A genome-wide transcriptional analysis of morphology determination in Candida albicans.Mol Biol Cell. 2013 Feb;24(3):246-60. doi: 10.1091/mbc.E12-01-0065. Epub 2012 Dec 14. Mol Biol Cell. 2013. PMID: 23242994 Free PMC article.
-
Cdc28-Cln3 phosphorylation of Sla1 regulates actin patch dynamics in different modes of fungal growth.Mol Biol Cell. 2012 Sep;23(17):3485-97. doi: 10.1091/mbc.E12-03-0231. Epub 2012 Jul 11. Mol Biol Cell. 2012. PMID: 22787279 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
