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. 2007 Dec;455(3):465-77.
doi: 10.1007/s00424-007-0314-3. Epub 2007 Jul 24.

Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1

Alexander Dietrich  1 , Hermann Kalwa, Ursula Storch, Michael Mederos y Schnitzler, Birgit Salanova, Olaf Pinkenburg, Galyna Dubrovska, Kirill Essin, Maik Gollasch, Lutz Birnbaumer, Thomas Gudermann
Affiliations

Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1

Alexander Dietrich et al. Pflugers Arch. 2007 Dec.

Abstract

Among the classical transient receptor potential (TRPC) subfamily, TRPC1 is described as a mechanosensitive and store-operated channel proposed to be activated by hypoosmotic cell swelling and positive pipette pressure as well as regulated by the filling status of intracellular Ca(2+) stores. However, evidence for a physiological role of TRPC1 may most compellingly be obtained by the analysis of a TRPC1-deficient mouse model. Therefore, we have developed and analyzed TRPC1(-/-) mice. Pressure-induced constriction of cerebral arteries was not impaired in TRPC1(-/-) mice. Smooth muscle cells from cerebral arteries activated by hypoosmotic swelling and positive pipette pressure showed no significant differences in cation currents compared to wild-type cells. Moreover, smooth muscle cells of TRPC1(-/-) mice isolated from thoracic aortas and cerebral arteries showed no change in store-operated cation influx induced by thapsigargin, inositol-1,4,5 trisphosphate, and cyclopiazonic acid compared to cells from wild-type mice. In contrast to these results, small interference RNAs decreasing the expression of stromal interaction molecule 1 (STIM1) inhibited thapsigargin-induced store-operated cation influx, demonstrating that STIM1 and TRPC1 are mutually independent. These findings also imply that, as opposed to current concepts, TRPC1 is not an obligatory component of store-operated and stretch-activated ion channel complexes in vascular smooth muscle cells.

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References

    1. Am J Physiol Heart Circ Physiol. 2006 Dec;291(6):H2653-9 - PubMed
    1. Nat Cell Biol. 2006 Sep;8(9):1003-10 - PubMed
    1. J Biol Chem. 2006 Jul 28;281(30):20661-5 - PubMed
    1. Circ Res. 2006 Mar 3;98(4):557-63 - PubMed
    1. Nature. 2007 Mar 15;446(7133):284-7 - PubMed

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