Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences
- PMID: 176427
- PMCID: PMC515442
- DOI: 10.1128/JVI.17.2.503-512.1976
Anatomy of herpes simplex virus DNA. V. Terminally repetitive sequences
Abstract
Native DNA from four strains of herpes simplex virus 1 (HSV-1) circularized after digestion with the lambda exonuclease, indicating that the molecules were terminally repetitious. In two strains, the terminal repetition was evident in nearly 50% of the DNA molecules. Maximal circularization was observed when only 0.25 to 0.5% of the DNA was depolymerized by the exonuclease, suggesting that the minimal size of the terminally repetitious regions is in the range of 400 to 800 bases pairs. More extensive exonuclease treatment resulted in a reduction in the frequency of circularization. To determine whether the terminally repetitive regions themselves contained self-annealing sequences that were precluding circularization of more extensively digested DNA, the terminal fragments from HinIII restriction endonuclease digests were isolated, denatured, and tested for their ability to self-anneal. The results of hydroxyapatite column chromatography and electron microscope examination of the terminal regions are consistent with this hypothesis.
Similar articles
-
A nearby inverted repeat of the terminal sequence of herpes simplex virus DNA.Biochem Biophys Res Commun. 1976 Jan 26;68(2):609-15. doi: 10.1016/0006-291x(76)91189-x. Biochem Biophys Res Commun. 1976. PMID: 175798 No abstract available.
-
Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components.Proc Natl Acad Sci U S A. 1975 Nov;72(11):4243-7. doi: 10.1073/pnas.72.11.4243. Proc Natl Acad Sci U S A. 1975. PMID: 172900 Free PMC article.
-
DNA of Epstein-Barr virus. V. Direct repeats of the ends of Epstein-Barr virus DNA.J Virol. 1979 Jun;30(3):852-62. doi: 10.1128/JVI.30.3.852-862.1979. J Virol. 1979. PMID: 225546 Free PMC article.
-
The terminal repetition of herpes simplex virus DNA.Virology. 1975 Sep;67(1):144-57. doi: 10.1016/0042-6822(75)90412-2. Virology. 1975. PMID: 169623 No abstract available.
-
Sequence arrangement in herpes simplex virus type 1 DNA: identification of terminal fragments in restriction endonuclease digests and evidence for inversions in redundant and unique sequences.J Virol. 1976 Oct;20(1):211-21. doi: 10.1128/JVI.20.1.211-221.1976. J Virol. 1976. PMID: 185412 Free PMC article.
Cited by
-
Appearance in vivo of single-stranded complementary ends on parental herpesvirus DNA.Proc Natl Acad Sci U S A. 1976 Aug;73(8):2674-8. doi: 10.1073/pnas.73.8.2674. Proc Natl Acad Sci U S A. 1976. PMID: 183205 Free PMC article.
-
Herpes simplex virus type 1 HindIII fragment L encodes spliced and complementary mRNA species.J Virol. 1981 Aug;39(2):559-72. doi: 10.1128/JVI.39.2.559-572.1981. J Virol. 1981. PMID: 6268843 Free PMC article.
-
Co-opting the Fanconi anemia genomic stability pathway enables herpesvirus DNA synthesis and productive growth.Mol Cell. 2014 Jul 3;55(1):111-22. doi: 10.1016/j.molcel.2014.05.020. Epub 2014 Jun 19. Mol Cell. 2014. PMID: 24954902 Free PMC article.
-
Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component.J Virol. 1976 Nov;20(2):527-31. doi: 10.1128/JVI.20.2.527-531.1976. J Virol. 1976. PMID: 185428 Free PMC article.
-
A model for replication of the ends of linear chromosomes.Nucleic Acids Res. 1976 Nov;3(11):3167-71. doi: 10.1093/nar/3.11.3167. Nucleic Acids Res. 1976. PMID: 188017 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
