Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 May 24;8(2):Article 39.
doi: 10.1208/pt0802039.

Chitosan film containing fucoidan as a wound dressing for dermal burn healing: preparation and in vitro/in vivo evaluation

Ali Demir Sezer  1 , Fatih Hatipoğlu, Erdal Cevher, Zeki Oğurtan, Ahmet Levent Baş, Jülide Akbuğa
Affiliations

Chitosan film containing fucoidan as a wound dressing for dermal burn healing: preparation and in vitro/in vivo evaluation

Ali Demir Sezer et al. AAPS PharmSciTech. .

Abstract

The aim of this study was to develop chitosan film containing fucoidan and to investigate its suitability for the treatment of dermal burns on rabbits. Porous films, thickness between 29.7 and 269.0 mum, were obtained by the solvent dropping method. Water vapor permeability (3.3-16.6/0.1 g), the swelling (0.67-1.77 g/g), tensile strength (7.1-45.8 N), and bioadhesion (0.076-1.771 mJ/cm(2)) of the films were determined. The thinnest films were obtained with the lowest chitosan concentration (P < .05). The water absorption capacity of the films sharply increased with the freeze-drying technique. The film having the thickness of 29.7 mum showed the highest amount of moisture permeability (16.6 g/0.1 g). Higher chitosan concentration significantly increased tensile strength of the films (P < .05). Using higher concentration of lactic acid made films more elastic and applicable, and these films were selected for in vivo studies. Seven adult male New Zealand white rabbits were used for the evaluation of the films on superficial dermal burns. Biopsy samples were taken at 7, 14, and 21 days after wounding, and each wound site was examined macroscopically and histopathologically. After 7 days treatment, fibroplasia and scar were observed on wounds treated with fucoidan-chitosan film. The best regenerated dermal papillary formation, best re-epithelization, and the fastest closure of wounds were found in the fucoidan-chitosan film treatment group after 14 days compared with other treatment and control groups. It can be concluded that fucoidan-chitosan films might be a potential treatment system for dermal burns and that changing formulation variables can modulate the characterizations of the films.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Park JE, Barbul A. Understanding the role of immune regulation in wound healing. Am J Surg. 2004;187:S11–S16. doi: 10.1016/S0002-9610(03)00296-4. - DOI - PubMed
    1. Shakespeare P. Burn wound healing and skin substitutes. Burns. 2001;27:517–522. doi: 10.1016/S0305-4179(01)00017-1. - DOI - PubMed
    1. Lloyd LL, Kennedy JF, Methacanon P, Paterson M, Knill CJ. Carbohydrate polymers as wound management aids. Carbohydr Polym. 1998;37:315–322. doi: 10.1016/S0144-8617(98)00077-0. - DOI
    1. Alemdaroğlu C, Değim Z, Çelebi N, Zor F, Öztürk S, Erdoğan D. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns. 2006;32:319–327. doi: 10.1016/j.burns.2005.10.015. - DOI - PubMed
    1. Ueno H, Murakami M, Okumura M, Kadosawa T, Uede T, Fujinaga T. Chitosan accelerates the production of osteopontin from polymorphonuclear leukocytes. Biomaterials. 2001;22:1667–1673. doi: 10.1016/S0142-9612(00)00328-8. - DOI - PubMed

Publication types

LinkOut - more resources