doi: 10.1038/nmeth1070.
Epub 2007 Jul 8.
Red-shifted Renilla reniformis luciferase variants for imaging in living subjects
Affiliations
- PMID: 17618292
- DOI: 10.1038/nmeth1070
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Red-shifted Renilla reniformis luciferase variants for imaging in living subjects
Nat Methods.
2007 Aug.
Abstract
The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.
Comment in
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Bringing bioluminescence into the picture.Nat Methods. 2007 Aug;4(8):616-7. doi: 10.1038/nmeth0807-616. Nat Methods. 2007. PMID: 17664945 No abstract available.
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