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Review
. 2007 Aug;8(8):645-54.
doi: 10.1038/nrm2208.

Analysis of protein complexes using mass spectrometry

Anne-Claude Gingras  1 , Matthias Gstaiger, Brian Raught, Ruedi Aebersold
Affiliations
Review

Analysis of protein complexes using mass spectrometry

Anne-Claude Gingras et al. Nat Rev Mol Cell Biol. 2007 Aug.

Abstract

The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.

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