Ectopic expression of a recessive resistance gene generates dominant potyvirus resistance in plants
- PMID: 17511813
- DOI: 10.1111/j.1467-7652.2007.00262.x
Ectopic expression of a recessive resistance gene generates dominant potyvirus resistance in plants
Abstract
Despite long-standing plant breeding investments and early successes in genetic engineering, plant viral pathogens still cause major losses in agriculture worldwide.Early transgenic approaches involved the expression of pathogen-derived sequences that provided limited protection against relatively narrow ranges of viral pathotypes. In contrast,this study demonstrates that the ectopic expression of pvr1, a recessive gene from Capsicum chinense, results in dominant broad-spectrum potyvirus resistance in transgenic tomato plants (Solanum lycopersicum). The pvr1 locus in pepper encodes the eukaryotic translation initiation factor eIF4E. Naturally occurring point mutations at this locus result in monogenic recessive broad-spectrum potyvirus resistance that has been globally deployed via plant breeding programmes for more than 50 years. Transgenic tomato progenies that over-expressed the Capsicum pvr1 allele showed dominant resistance to several tobacco etch virus strains and other potyviruses, including pepper mottle virus, a range of protection similar to that observed in pepper homozygous for the pvr1 allele.
Similar articles
-
Engineering virus resistance using a modified potato gene.Plant Biotechnol J. 2011 Dec;9(9):1014-21. doi: 10.1111/j.1467-7652.2011.00622.x. Epub 2011 Jun 12. Plant Biotechnol J. 2011. PMID: 21668622
-
The pvr1 locus in Capsicum encodes a translation initiation factor eIF4E that interacts with Tobacco etch virus VPg.Plant J. 2005 May;42(3):392-405. doi: 10.1111/j.1365-313X.2005.02381.x. Plant J. 2005. PMID: 15842624
-
Tobacco etch virus infectivity in Capsicum spp. is determined by a maximum of three amino acids in the viral virulence determinant VPg.Mol Plant Microbe Interact. 2012 Dec;25(12):1562-73. doi: 10.1094/MPMI-04-12-0091-R. Mol Plant Microbe Interact. 2012. PMID: 23134519
-
Recessive resistance to plant viruses.Adv Virus Res. 2009;75:119-59. doi: 10.1016/S0065-3527(09)07504-6. Epub 2010 Jan 13. Adv Virus Res. 2009. PMID: 20109665 Review.
-
Toward a quarter century of pathogen-derived resistance and practical approaches to plant virus disease control.Adv Virus Res. 2009;75:161-83. doi: 10.1016/S0065-3527(09)07505-8. Epub 2010 Jan 13. Adv Virus Res. 2009. PMID: 20109666 Review.
Cited by
-
Molecular and cellular control of cell death and defense signaling in pepper.Planta. 2015 Jan;241(1):1-27. doi: 10.1007/s00425-014-2171-6. Epub 2014 Sep 25. Planta. 2015. PMID: 25252816 Review.
-
Plant Translation Factors and Virus Resistance.Viruses. 2015 Jun 24;7(7):3392-419. doi: 10.3390/v7072778. Viruses. 2015. PMID: 26114476 Free PMC article. Review.
-
Genome Editing of eIF4E1 in Tomato Confers Resistance to Pepper Mottle Virus.Front Plant Sci. 2020 Jul 24;11:1098. doi: 10.3389/fpls.2020.01098. eCollection 2020. Front Plant Sci. 2020. PMID: 32849681 Free PMC article.
-
Eukaryotic translation initiation factor 4E-mediated recessive resistance to plant viruses and its utility in crop improvement.Mol Plant Pathol. 2012 Sep;13(7):795-803. doi: 10.1111/j.1364-3703.2012.00791.x. Epub 2012 Mar 2. Mol Plant Pathol. 2012. PMID: 22379950 Free PMC article. Review.
-
Manipulating Cellular Factors to Combat Viruses: A Case Study From the Plant Eukaryotic Translation Initiation Factors eIF4.Front Microbiol. 2019 Feb 5;10:17. doi: 10.3389/fmicb.2019.00017. eCollection 2019. Front Microbiol. 2019. PMID: 30804892 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
