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Review
. 2007 May;158(2):244-53.
doi: 10.1016/j.jsb.2007.04.001. Epub 2007 Apr 10.

Looking into live cells with in-cell NMR spectroscopy

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Review

Looking into live cells with in-cell NMR spectroscopy

Philipp Selenko et al. J Struct Biol. 2007 May.

Abstract

In-cell NMR spectroscopy has gained recent popularity since it provides means to analyze the conformational and functional properties of proteins inside living cells and at atomic resolution. High-resolution in-cell NMR spectroscopy was originally established in bacterial cells and based on a rationale that relies on protein over-expression and sample analysis within the same cellular environment. Here, we review in-cell NMR approaches in Xenopus laevis oocytes and evaluate potential future applications in other eukaryotic cell types.

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