Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1991 Dec;173(24):7828-33.
doi: 10.1128/jb.173.24.7828-7833.1991.

Genetic evidence for interaction of sigma E with the spoIIID promoter in Bacillus subtilis

K M Tatti  1 , C H Jones, C P Moran Jr
Affiliations

Genetic evidence for interaction of sigma E with the spoIIID promoter in Bacillus subtilis

K M Tatti et al. J Bacteriol. 1991 Dec.

Abstract

During sporulation in Bacillus subtilis, new RNA polymerase sigma factors are produced. These sigma factors direct the transcription of genes that are required for this cellular differentiation. In order to determine the role of each sigma factor in this process, it is necessary to know which promoters are recognized by each sigma factor. The spoIIID gene product plays an important role in the establishment of mother cell-specific gene expression during sporulation. We found that substitution of an alanine at position 124 of the sporulation-specific sigma factor sigma E suppressed the effect of a single-base-pair transition at position -13 of the spoIIID promoter. This alanine substitution in sigma E did not suppress the effect of a transversion at position -12 of the spoIIID promoter. The allele specificity of the interaction between sigma E and the spoIIID promoter is strong evidence that sigma E directs transcription from the spoIIID promoter during sporulation. Position 124 in sigma E is located within a region that is highly conserved among the regions in other sigma factors that probably interact with the -10 regions of their cognate promoters.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4533-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Sep;54(3):704-11 - PubMed
    1. J Mol Biol. 1990 Sep 20;215(2):267-76 - PubMed
    1. Mol Microbiol. 1991 Jun;5(6):1363-73 - PubMed
    1. J Bacteriol. 1991 Jun;173(11):3282-90 - PubMed

Publication types

LinkOut - more resources