Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Feb 15;89(4):1291-5.
doi: 10.1073/pnas.89.4.1291.

Myb and Ets proteins cooperate in transcriptional activation of the mim-1 promoter

H Dudek  1 , R V Tantravahi, V N Rao, E S Reddy, E P Reddy
Affiliations

Myb and Ets proteins cooperate in transcriptional activation of the mim-1 promoter

H Dudek et al. Proc Natl Acad Sci U S A. .

Abstract

In the generation of the acutely transforming avian retrovirus E26, both myb and ets genes have been transduced, leading to the production of a Gag-Myb-Ets fusion protein. This co-occurrence of v-myb and v-ets oncogenes suggests that the two might have a functional relationship. To look for such a relationship, we tested the transcriptional activation activity of Myb alone or with coexpressed Ets-1 or Ets-2. Using the promoter of the v-Myb-inducible mim-1 gene as a target, we found that full-length c-Myb gene products were poor activators of transcription, while an oncogenic (truncated) form of this protein was a strong trans-activator. However, coexpression of Ets-2 with full-length or truncated forms of Myb greatly increased trans-activation. Coexpression of Ets-1, Fos, Jun, or Myc with Myb did not increase trans-activation of the mim-1 promoter. The ability of Myb and Ets-2 to transactivate was cooperative, since Ets-2 alone gave little or no activation. Bacterially synthesized Ets-2 protein was found to bind specifically to the mim-1 promoter, suggesting that it may be a target for both Myb and Ets proteins. Thus, Myb and Ets proteins can cooperate in transcriptional activation, and their co-occurrence in the E26 virus may reflect a functional relationship between these two oncoproteins. Truncated forms of Myb may have a reduced need for cooperating factors such as Ets-2, and this might constitute an important mechanism associated with oncogenic activation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Cell. 1986 Sep 26;46(7):973-82 - PubMed
    1. Oncogene. 1987;2(1):79-84 - PubMed
    1. Nature. 1986 Feb 13-19;319(6054):604-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7232-6 - PubMed
    1. Oncogene. 1990 May;5(5):657-61 - PubMed

Publication types

MeSH terms

LinkOut - more resources