Comparative Study
doi: 10.1177/1087057107300221.
Epub 2007 Apr 4.
Development of a Transcreener kinase assay for protein kinase A and demonstration of concordance of data with a filter-binding assay format
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- PMID: 17409274
- DOI: 10.1177/1087057107300221
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Comparative Study
Development of a Transcreener kinase assay for protein kinase A and demonstration of concordance of data with a filter-binding assay format
J Biomol Screen.
2007 Jun.
Abstract
A Transcreener kinase fluorescence polarization (FP) assay has been developed for the serine/threonine kinase protein kinase A (PKA). The PKA Transcreener kinase assay is an homogenous, competitive antibody-based FP assay that uses Far Red Alexa Fluor 633-labeled adenosine 5' disphosphate (ADP) tracer and mouse monoclonal anti-ADP antibody. The Transcreener PKA assay was validated with both known PKA inhibitors and library compounds. The Transcreener PKA assay is resistant to low-wavelength (or common) fluorescent interference from small-molecule library compounds and generates IC50 results comparable with current radioactive filter-binding assay.
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