doi: 10.1038/nprot.2006.239.
Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol
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- PMID: 17406450
- DOI: 10.1038/nprot.2006.239
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Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol
Nat Protoc.
2006.
Abstract
The telomeric repeat amplification protocol (TRAP) is a two-step process for analyzing telomerase activity in cell or tissue extracts. Recent modifications of this sensitive assay include elimination of radioactivity by using a fluorescently labeled primer instead of a radiolabeled primer. In addition, the TRAP assay has been modified for real-time, quantitative PCR analysis. Here, we describe cost-effective procedures for detection of telomerase activity using a fluorescent-based assay as well as by using real-time PCR. These modified TRAP assays can be accomplished within 4 h (from lysis of samples to analysis of telomerase products).
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