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Review
. 2007 Mar 30;3(3):e42.
doi: 10.1371/journal.pcbi.0030042.

Deciphering protein-protein interactions. Part I. Experimental techniques and databases

Benjamin A Shoemaker  1 , Anna R Panchenko
Affiliations
Review

Deciphering protein-protein interactions. Part I. Experimental techniques and databases

Benjamin A Shoemaker et al. PLoS Comput Biol. .
No abstract available

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Conflict of interest statement

Competing interests. The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Schematic Representations of Main Experimental Techniques Used for High-Throughput Analysis of Protein Interactions
(A) Y2H detects interactions between proteins X and Y, where X is linked to BD domain which binds to upstream activating sequence (UAS) of a promoter. (B) MS identifies polypeptide sequence. (C) TAP purifies protein complexes and removes the molecules of contaminants. (D) Gene coexpression analysis produces the correlation matrix where the dark areas show high correlation between expression levels of corresponding genes. (E) Protein microarrays (protein chips) can detect interactions between actual proteins rather than genes: target proteins immobilized on the solid support are probed with a fluorescently labeled protein. (F) Synthetic lethality method describes the genetic interaction when two individual, nonlethal mutations result in lethality when administered together (a− b−).
See this image and copyright information in PMC

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