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. 2007 May;50(3):414-28.
doi: 10.1111/j.1365-313X.2007.03056.x. Epub 2007 Mar 21.

Structural analogs of ABA reveal novel features of ABA perception and signaling in Arabidopsis

Daiqing Huang  1 , Masrur R Jaradat, Weiren Wu, Stephen J Ambrose, Andrew R Ross, Suzanne R Abrams, Adrian J Cutler
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Free article

Structural analogs of ABA reveal novel features of ABA perception and signaling in Arabidopsis

Daiqing Huang et al. Plant J. 2007 May.
Free article

Abstract

Changes in gene expression produced by the application of (+)-abscisic acid (ABA) to Arabidopsis thaliana plants were compared with changes produced by the ABA structural analogs (-)-ABA, (+)-8'-acetylene ABA and (-)-2',3'-dihydroacetylenic abscisyl alcohol. The maximum expression of many rapidly (+)-ABA-induced genes occurred prior to peak hormone accumulation, suggesting negative feedback regulation that may be mediated by the induction of genes encoding PP2C-type protein phosphatases. For most rapidly (+)-ABA-induced genes, expression was delayed in ABA analog treatments although analogs accumulated to higher levels than did (+)-ABA. For each analog, some genes exhibited a hypersensitive response to the analog and some genes were less sensitive to the analog than to (+)-ABA. Variations in the sensitivity of gene expression to (+)-ABA and analogs reflect the different structural requirements of two or more classes of hormone receptors. By using ABA analogs to reveal and confirm weakly (+)-ABA-regulated genes, we estimate that 14% of Arabidopsis genes are ABA-regulated in aerial tissues. Treatments with the analog (+)-8'-acetylene ABA (PBI425) led to the identification of new ABA-regulated genes. As an example, the transcription factor MYBR1 was significantly induced by PBI425, but not by (+)-ABA, and is shown to play a role in ABA signaling by phenotypic analysis of gain-of-function and loss-of-function mutants.

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