Role of an acidic cluster/dileucine motif in cation-independent mannose 6-phosphate receptor traffic
- PMID: 17319895
- DOI: 10.1111/j.1600-0854.2007.00541.x
Role of an acidic cluster/dileucine motif in cation-independent mannose 6-phosphate receptor traffic
Abstract
The endocytic trafficking of the cation-independent mannose 6-phosphate receptor (CI-MPR) involves multiple sorting steps. A cluster of acidic amino acids followed by a dileucine motif in the cytoplasmic tail has been proposed to mediate receptor sorting from the trans Golgi network (TGN) to late endosomes. Mutations in this motif impair lysosomal enzyme sorting by preventing association of CI-MPR with coat proteins. The role of the acidic cluster/dileucine motif in the post-endocytic transport of the receptor was examined using the CI-MPR mutants, AC01 and D160E (Chen HJ, Yuan J, Lobel P. J Biol Chem 1997;272:7003-7012). Following internalization, wild type (WT) CI-MPR is transported through sorting endosomes into the endocytic recycling compartment (ERC), after which it traffics to the TGN and other organelles. However, the mutants localize mostly to the ERC and only a small portion reaches the TGN, suggesting that the sorting of the CI-MPR mutants from the ERC into the TGN is severely impaired. We observed no defect in receptor internalization or in the rate of tail mutant recycling to the cell surface compared to the WT. These results demonstrate that the acidic cluster/dileucine motif of CI-MPR is critical for receptor sorting at early stages of intracellular transport following endocytosis.
Similar articles
-
Binding of GGA2 to the lysosomal enzyme sorting motif of the mannose 6-phosphate receptor.Science. 2001 Jun 1;292(5522):1716-8. doi: 10.1126/science.1060896. Science. 2001. PMID: 11387476
-
Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes.Mol Biol Cell. 2004 Feb;15(2):721-33. doi: 10.1091/mbc.e03-07-0497. Epub 2003 Oct 31. Mol Biol Cell. 2004. PMID: 14595110 Free PMC article.
-
Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor.J Cell Biol. 2004 Apr;165(1):123-33. doi: 10.1083/jcb.200312055. J Cell Biol. 2004. PMID: 15078903 Free PMC article.
-
Post-Golgi traffic in plants.Traffic. 2009 Jul;10(7):819-28. doi: 10.1111/j.1600-0854.2009.00916.x. Epub 2009 Apr 18. Traffic. 2009. PMID: 19416470 Review.
-
Mannose 6-phosphate receptors in sorting and transport of lysosomal enzymes.Biochim Biophys Acta. 1995 Jul 17;1241(2):177-94. doi: 10.1016/0304-4157(95)00004-b. Biochim Biophys Acta. 1995. PMID: 7640295 Review.
Cited by
-
Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism.Exp Cell Res. 2008 Jul 1;314(11-12):2249-56. doi: 10.1016/j.yexcr.2008.04.003. Epub 2008 Apr 12. Exp Cell Res. 2008. PMID: 18501893 Free PMC article.
-
Regulation of post-Golgi traffic of G protein-coupled receptors.Subcell Biochem. 2012;63:83-95. doi: 10.1007/978-94-007-4765-4_5. Subcell Biochem. 2012. PMID: 23161134 Free PMC article. Review.
-
Contribution of endocytic motifs in the cytoplasmic tail of herpes simplex virus type 1 glycoprotein B to virus replication and cell-cell fusion.J Virol. 2007 Dec;81(24):13889-903. doi: 10.1128/JVI.01231-07. Epub 2007 Oct 3. J Virol. 2007. PMID: 17913800 Free PMC article.
-
Rapid Clathrin-Mediated Uptake of Recombinant α-Gal-A to Lysosome Activates Autophagy.Biomolecules. 2020 May 30;10(6):837. doi: 10.3390/biom10060837. Biomolecules. 2020. PMID: 32486191 Free PMC article.
-
Intracellular trafficking of LRP9 is dependent on two acidic cluster/dileucine motifs.Histochem Cell Biol. 2008 Aug;130(2):315-27. doi: 10.1007/s00418-008-0436-5. Epub 2008 May 7. Histochem Cell Biol. 2008. PMID: 18461348
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
