Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting
- PMID: 1730622
Molecular characterization of VAC1, a gene required for vacuole inheritance and vacuole protein sorting
Abstract
Cell division requires an accurate partitioning of cytoplasmic organelles. The segregation of vacuoles in the budding yeast Saccharomyces cerevisaie occurs at a specific time in the cell cycle and is spatially targeted to the small bud. Several yeast vac mutants have been isolated which are defective in this process. We have now cloned the VAC1 gene, corresponding to the first of these mutants, vac1-1. This gene encodes a protein of 515 amino acids, without homolog in the current data bases. It contains neither long hydrophobic stretches nor a classical leader peptide. The most notable aspect of the sequence is the presence of three zinc fingers. Yeast in which the VAC1 gene has been entirely deleted are viable. However, they grow more slowly than wild-type cells and only form microcolonies when grown on glycerol at 37 degrees C. These yeast are defective in vacuole segregation at both the permissive and nonpermissive temperatures. The vac1 mutant was previously shown to mislocalize carboxypeptidase Y to the cell surface, suggesting that Vac1p is involved in more than one vesicular traffic pathway.
Similar articles
-
A novel Sec18p/NSF-dependent complex required for Golgi-to-endosome transport in yeast.Mol Biol Cell. 1997 Jun;8(6):1089-104. doi: 10.1091/mbc.8.6.1089. Mol Biol Cell. 1997. PMID: 9201718 Free PMC article.
-
A putative zinc finger protein, Saccharomyces cerevisiae Vps18p, affects late Golgi functions required for vacuolar protein sorting and efficient alpha-factor prohormone maturation.Mol Cell Biol. 1991 Dec;11(12):5813-24. doi: 10.1128/mcb.11.12.5813-5824.1991. Mol Cell Biol. 1991. PMID: 1840635 Free PMC article.
-
The yeast VPS17 gene encodes a membrane-associated protein required for the sorting of soluble vacuolar hydrolases.J Biol Chem. 1993 Jan 5;268(1):559-69. J Biol Chem. 1993. PMID: 8416961
-
Vacuolar sorting. Tracking down an elusive receptor.Curr Biol. 1994 Nov 1;4(11):1019-22. doi: 10.1016/s0960-9822(00)00231-1. Curr Biol. 1994. PMID: 7874484 Review.
-
Sorting of membrane proteins in the yeast secretory pathway.J Biol Chem. 1994 Apr 8;269(14):10185-8. J Biol Chem. 1994. PMID: 8144594 Review. No abstract available.
Cited by
-
Molecular aspects of the endocytic pathway.Biochem J. 1998 Dec 1;336 ( Pt 2)(Pt 2):271-82. doi: 10.1042/bj3360271. Biochem J. 1998. PMID: 9820800 Free PMC article. Review.
-
Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro.J Cell Biol. 1994 Jul;126(1):99-110. doi: 10.1083/jcb.126.1.99. J Cell Biol. 1994. PMID: 8027190 Free PMC article.
-
Cdc1 and the vacuole coordinately regulate Mn2+ homeostasis in the yeast Saccharomyces cerevisiae.Genetics. 1998 Apr;148(4):1787-98. doi: 10.1093/genetics/148.4.1787. Genetics. 1998. PMID: 9560393 Free PMC article.
-
A conserved regulatory mode in exocytic membrane fusion revealed by Mso1p membrane interactions.Mol Biol Cell. 2013 Feb;24(3):331-41. doi: 10.1091/mbc.E12-05-0415. Epub 2012 Nov 28. Mol Biol Cell. 2013. PMID: 23197474 Free PMC article.
-
In vitro reactions of vacuole inheritance in Saccharomyces cerevisiae.J Cell Biol. 1992 Dec;119(6):1469-79. doi: 10.1083/jcb.119.6.1469. J Cell Biol. 1992. PMID: 1334958 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
