Lesion bypass activity of DNA polymerase A from the extremely radioresistant organism Deinococcus radiodurans
- PMID: 17303573
- DOI: 10.1074/jbc.M611404200
Lesion bypass activity of DNA polymerase A from the extremely radioresistant organism Deinococcus radiodurans
Abstract
The bacterium Deinococcus radiodurans survives extremely high exposure to ionizing radiation and extended periods of desiccation. Radiation at the survival doses is known to cause numerous DNA damage, such as hundreds of double strand breaks and single strand breaks, as well as damage of the nucleobases. The mechanisms of D. radiodurans to survive the depicted threats are still only beginning to be understood. DNA polymerase A (PolA) has been shown to be crucially involved in irradiation resistance mechanisms of D. radiodurans. We expressed and characterized the DNA polymerase domain of PolA for the first time in vitro. The obtained enzyme is able to efficiently catalyze DNA-dependent DNA synthesis requiring Mg(II) as divalent metal ion. Additionally, strand displacement synthesis of the DNA polymerase, which is required in several repair processes, could be detected. We further found that DNA polymerase function of PolA is modulated by the presence of Mn(II). Whereas proceeding DNA synthesis of PolA was blocked by certain DNA damage that occurs through radiation of DNA, bypass was facilitated by Mn(II). Our results suggest an enzyme modulator function of Mn(II). These observations parallel reports that D. radiodurans accumulates intracellular Mn(II) in cases of irradiation and that the level of irradiation protection correlates with Mn(II) concentrations.
Similar articles
-
DNA polymerase X from Deinococcus radiodurans implicated in bacterial tolerance to DNA damage is characterized as a short patch base excision repair polymerase.Microbiology (Reading). 2009 Sep;155(Pt 9):3005-3014. doi: 10.1099/mic.0.029223-0. Epub 2009 Jun 18. Microbiology (Reading). 2009. PMID: 19542005
-
Enzymes involved in DNA ligation and end-healing in the radioresistant bacterium Deinococcus radiodurans.BMC Mol Biol. 2007 Aug 16;8:69. doi: 10.1186/1471-2199-8-69. BMC Mol Biol. 2007. PMID: 17705817 Free PMC article.
-
DNA polymerase X from Deinococcus radiodurans possesses a structure-modulated 3'-->5' exonuclease activity involved in radioresistance.Mol Microbiol. 2006 Apr;60(1):165-76. doi: 10.1111/j.1365-2958.2006.05077.x. Mol Microbiol. 2006. PMID: 16556228
-
DNA repair in the extremely radioresistant bacterium Deinococcus radiodurans.Mol Microbiol. 1994 Jul;13(1):9-15. doi: 10.1111/j.1365-2958.1994.tb00397.x. Mol Microbiol. 1994. PMID: 7984097 Review.
-
Deinococcus radiodurans - the consummate survivor.Nat Rev Microbiol. 2005 Nov;3(11):882-92. doi: 10.1038/nrmicro1264. Nat Rev Microbiol. 2005. PMID: 16261171 Review.
Cited by
-
The DnaE polymerase from Deinococcus radiodurans features RecA-dependent DNA polymerase activity.Biosci Rep. 2016 Dec 5;36(6):e00419. doi: 10.1042/BSR20160364. Print 2016 Dec. Biosci Rep. 2016. PMID: 27789781 Free PMC article.
-
Lineage-specific variations in the trigger loop modulate RNA proofreading by bacterial RNA polymerases.Nucleic Acids Res. 2016 Feb 18;44(3):1298-308. doi: 10.1093/nar/gkv1521. Epub 2016 Jan 4. Nucleic Acids Res. 2016. PMID: 26733581 Free PMC article.
-
Oxidative stress resistance in Deinococcus radiodurans.Microbiol Mol Biol Rev. 2011 Mar;75(1):133-91. doi: 10.1128/MMBR.00015-10. Microbiol Mol Biol Rev. 2011. PMID: 21372322 Free PMC article. Review.
-
Gre-family factors modulate DNA damage sensing by Deinococcus radiodurans RNA polymerase.RNA Biol. 2019 Dec;16(12):1711-1720. doi: 10.1080/15476286.2019.1656027. Epub 2019 Aug 21. RNA Biol. 2019. PMID: 31416390 Free PMC article.
-
Alternative bacterial two-component small heat shock protein systems.Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20407-12. doi: 10.1073/pnas.1209565109. Epub 2012 Nov 26. Proc Natl Acad Sci U S A. 2012. PMID: 23184973 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
