Immuno-isolation of Sec7p-coated transport vesicles from the yeast secretory pathway
- PMID: 1729652
- DOI: 10.1038/355173a0
Immuno-isolation of Sec7p-coated transport vesicles from the yeast secretory pathway
Abstract
The transport of proteins destined for post-endoplasmic reticulum locations in the secretory pathway is mediated by small vesicular carriers. Transport vesicles have been generated in cell-free assays from the yeast Saccharomyces cerevisiae, and mammalian systems. Yeast genes encoding cytosolic components that participate in vesicular traffic were first identified from the collection of conditional-lethal sec-(secretory) mutants. Mutations in the yeast SEC7 gene disrupt protein transport in the secretory pathway at the nonpermissive temperature. The SEC7 gene product is a phosphoprotein of relative molecular mass 230,000 that functions from the cytoplasmic aspect of intracellular membranes. We report that in a yeast cell-free transport assay, the introduction of antibodies to Sec7 protein (Sec7p) results in the accumulation of transport vesicles. These vesicles are retrieved with Sec7p-specific antibodies by immuno-isolation for biochemical and electron microscopic characterization. Sec7p on the surface of the accumulated transport vesicles, in combination with previous genetic and biochemical studies, implicate Sec7p as part of a (non-clathrin) vesicle coat. This Sec7p-containing coat structure is proposed to be essential for vesicle budding at multiple stages in the yeast secretory pathway.
Similar articles
-
Sec7p directs the transitions required for yeast Golgi biogenesis.Traffic. 2000 Feb;1(2):172-83. doi: 10.1034/j.1600-0854.2000.010209.x. Traffic. 2000. PMID: 11208097
-
Human ARF4 expression rescues sec7 mutant yeast cells.Mol Cell Biol. 1996 Jul;16(7):3275-84. doi: 10.1128/MCB.16.7.3275. Mol Cell Biol. 1996. PMID: 8668142 Free PMC article.
-
An N-end rule destabilization mutant reveals pre-Golgi requirements for Sec7p in yeast membrane traffic.Biochem Biophys Res Commun. 1998 Feb 4;243(1):191-8. doi: 10.1006/bbrc.1998.8084. Biochem Biophys Res Commun. 1998. PMID: 9473503
-
Protein secretion and compartmentalization in yeast.Microbiologia. 1988 Jun;4(2):73-85. Microbiologia. 1988. PMID: 3077331 Review.
-
Ultracytochemical evidence of Golgi functions in microvesicles at all phases of cell cycle in Saccharomyces cerevisiae.Micron. 1995;26(2):175-90. doi: 10.1016/0968-4328(95)00003-m. Micron. 1995. PMID: 7767635 Review.
Cited by
-
Membrane stress is coupled to a rapid translational control of gene expression in chlorpromazine-treated cells.Curr Genet. 2007 Sep;52(3-4):171-85. doi: 10.1007/s00294-007-0151-0. Epub 2007 Aug 21. Curr Genet. 2007. PMID: 17710403
-
Guanosine diphosphatase is required for protein and sphingolipid glycosylation in the Golgi lumen of Saccharomyces cerevisiae.J Cell Biol. 1993 Jul;122(2):307-23. doi: 10.1083/jcb.122.2.307. J Cell Biol. 1993. PMID: 8391537 Free PMC article.
-
Transport of proteins in eukaryotic cells: more questions ahead.Plant Mol Biol. 1996 Oct;32(1-2):223-49. doi: 10.1007/BF00039384. Plant Mol Biol. 1996. PMID: 8980481 Review.
-
Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae.Mol Biol Cell. 2004 Nov;15(11):5021-37. doi: 10.1091/mbc.e04-05-0411. Epub 2004 Sep 8. Mol Biol Cell. 2004. PMID: 15356266 Free PMC article.
-
A C-terminal sequence in the guanine nucleotide exchange factor Sec7 mediates Golgi association and interaction with the Rsp5 ubiquitin ligase.J Biol Chem. 2008 Dec 5;283(49):34188-96. doi: 10.1074/jbc.M806023200. Epub 2008 Oct 2. J Biol Chem. 2008. PMID: 18832381 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
