Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jun;133(6):389-99.
doi: 10.1007/s00432-006-0184-7. Epub 2007 Jan 12.

Increased induction of antitumor response by exosomes derived from interleukin-2 gene-modified tumor cells

Yunshan Yang  1 , Fangming Xiu, Zhijian Cai, Jianli Wang, Qingqing Wang, Yangxin Fu, Xuetao Cao
Affiliations

Increased induction of antitumor response by exosomes derived from interleukin-2 gene-modified tumor cells

Yunshan Yang et al. J Cancer Res Clin Oncol. 2007 Jun.

Abstract

Purpose: Tumor-derived exosomes (TEX) have been proposed as a new kind of cancer vaccine; however, their in vivo antitumor effects are not satisfactory. In order to further improve the efficacy of vaccination with TEX, we investigated whether interleukin-2 (IL-2) genetic modification of tumor cells can make IL-2 presence in the exosomes, thus increasing antitumor effects of the TEX.

Methods: E.G7-OVA tumor cells expressing Ovalbumin (OVA) as a tumor model antigen were used to prepare TEX by serial centrifugation and sucrose gradients ultracentrifugation. To demonstrate their antitumor effects, IL-2-containing exosomes (Exo/IL-2) were injected subcutaneously into C57BL/C mice: either bearing tumor or followed by tumor inoculation.

Results: We found IL-2 within those exosomes as detected by both ELISA and Western blot. Vaccination with these Exo/IL-2 could induce antigen-specific Th1-polarized immune response and Cytotoxic T lymphocytes (CTL) more efficiently, resulting in more significant inhibition of tumor growth. CD8(+) T cells are the main effector cells, however, CD4(+) T cells, and NK cells are also involved in the induction of antitumor response of this approach.

Conclusions: Our results demonstrate that IL-2 genetic modification of tumor cells can make the TEX contain IL-2 with the increased antitumor effects, representing a promising way of exosome-based tumor vaccine.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Lab Invest. 2006 Mar;86(3):231-45 - PubMed
    1. Nat Rev Immunol. 2002 Aug;2(8):569-79 - PubMed
    1. J Transl Med. 2005 Mar 02;3(1):10 - PubMed
    1. J Exp Clin Cancer Res. 1999 Jun;18(2):191-200 - PubMed
    1. J Exp Clin Cancer Res. 1999 Jun;18(2):173-9 - PubMed

Publication types

LinkOut - more resources