doi: 10.1016/j.chembiol.2006.10.005.
RNA-mediated sequestration of the RNA helicase eIF4A by Pateamine A inhibits translation initiation
Affiliations
- PMID: 17185224
- DOI: 10.1016/j.chembiol.2006.10.005
Item in Clipboard
RNA-mediated sequestration of the RNA helicase eIF4A by Pateamine A inhibits translation initiation
Chem Biol.
2006 Dec.
Abstract
Eukaryotic initiation factor 4A (eIF4A) is a member of the DEAD-box family of putative RNA helicases whose members are involved in many aspects of RNA metabolism. eIF4A is thought to facilitate binding of 43S preinitiation complexes to mRNAs by unwinding secondary structures present in the 5' untranslated region. Pateamine A, a small-molecule inhibitor of translation initiation, acts in an unusual manner by stimulating eIF4A activity. Herein, we report the elucidation of pateamine's mode of action. We demonstrate that Pateamine A is a chemical inducer of dimerization that forces an engagement between eIF4A and RNA and prevents eIF4A from participating in the ribosome-recruitment step of translation initiation.
Comment in
-
Translational dysregulation by Pateamine A.Chem Biol. 2007 Jan;14(1):5-7. doi: 10.1016/j.chembiol.2007.01.003. Chem Biol. 2007. PMID: 17254945 Free PMC article.
Similar articles
-
Inhibition of eukaryotic translation initiation by the marine natural product pateamine A.Mol Cell. 2005 Dec 9;20(5):709-22. doi: 10.1016/j.molcel.2005.10.008. Mol Cell. 2005. PMID: 16337595
-
Substrate-dependent targeting of eukaryotic translation initiation factor 4A by pateamine A: negation of domain-linker regulation of activity.Chem Biol. 2007 Jun;14(6):715-27. doi: 10.1016/j.chembiol.2007.05.012. Chem Biol. 2007. PMID: 17584618
-
Stopping trouble before it starts.ACS Chem Biol. 2006 Feb 17;1(1):17-9. doi: 10.1021/cb0600029. ACS Chem Biol. 2006. PMID: 17163634
-
Selective targeting of the DEAD-box RNA helicase eukaryotic initiation factor (eIF) 4A by natural products.Nat Prod Rep. 2020 May 1;37(5):609-616. doi: 10.1039/c9np00052f. Epub 2019 Nov 29. Nat Prod Rep. 2020. PMID: 31782447 Review.
-
Isolation and identification of eukaryotic initiation factor 4A as a molecular target for the marine natural product Pateamine A.Methods Enzymol. 2007;431:303-24. doi: 10.1016/S0076-6879(07)31014-8. Methods Enzymol. 2007. PMID: 17923240 Review.
Cited by
-
Mechanisms of repeat-associated non-AUG translation in neurological microsatellite expansion disorders.Biochem Soc Trans. 2021 Apr 30;49(2):775-792. doi: 10.1042/BST20200690. Biochem Soc Trans. 2021. PMID: 33729487 Free PMC article. Review.
-
From unwinding to clamping - the DEAD box RNA helicase family.Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):505-16. doi: 10.1038/nrm3154. Nat Rev Mol Cell Biol. 2011. PMID: 21779027 Review.
-
Regulation of translation initiation in eukaryotes: mechanisms and biological targets.Cell. 2009 Feb 20;136(4):731-45. doi: 10.1016/j.cell.2009.01.042. Cell. 2009. PMID: 19239892 Free PMC article. Review.
-
Stress Granules in Cancer.Rev Physiol Biochem Pharmacol. 2023;185:25-52. doi: 10.1007/112_2020_37. Rev Physiol Biochem Pharmacol. 2023. PMID: 32789791 Free PMC article.
-
Targeting the DEAD-Box RNA Helicase eIF4A with Rocaglates-A Pan-Antiviral Strategy for Minimizing the Impact of Future RNA Virus Pandemics.Microorganisms. 2021 Mar 5;9(3):540. doi: 10.3390/microorganisms9030540. Microorganisms. 2021. PMID: 33807988 Free PMC article. Review.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous
