microRNA-mediated silencing inside P-bodies
- PMID: 17179742
- DOI: 10.4161/rna.3.3.3499
microRNA-mediated silencing inside P-bodies
Abstract
Cytoplasmic processing bodies, or P-bodies, contain a high concentration of enzymes and factors required for mRNA turnover and translational repression. Recent studies provide evidence that the mRNAs silenced by miRNAs are localized to P-bodies for storage or degradation, perhaps in adjacent subcompartments. mRNP remodeling, potentially induced by miRISC or RNA helicase activity, may cause the modification of the translation initiation complex at the 5' end of mRNA, following translational repression and localization to P-bodies. Further remodeling in P-bodies may facilitate access of the decapping complex to the cap structure, thus inducing mRNA degradation. However, with appropriate signals, stored mRNAs in P-bodies could be released and returned to the translational machinery through mechanisms requiring binding of regulatory proteins to the 3' UTR of mRNAs. Here a model is proposed to explain the repression and degradation stages of the mRNAs within PBs. This model includes preservation or disruption of a stable closed loop structure of the mRNAs, compartmentalization in PBs and mRNA escape triggered by additional binding proteins.
Similar articles
-
P-body formation is a consequence, not the cause, of RNA-mediated gene silencing.Mol Cell Biol. 2007 Jun;27(11):3970-81. doi: 10.1128/MCB.00128-07. Epub 2007 Apr 2. Mol Cell Biol. 2007. PMID: 17403906 Free PMC article.
-
General translational repression by activators of mRNA decapping.Cell. 2005 Sep 23;122(6):875-86. doi: 10.1016/j.cell.2005.07.012. Cell. 2005. PMID: 16179257 Free PMC article.
-
P-bodies react to stress and nonsense.Cell. 2006 Jun 16;125(6):1036-8. doi: 10.1016/j.cell.2006.06.003. Cell. 2006. PMID: 16777595
-
RNA decapping inside and outside of processing bodies.Curr Opin Cell Biol. 2005 Jun;17(3):326-31. doi: 10.1016/j.ceb.2005.04.002. Curr Opin Cell Biol. 2005. PMID: 15901504 Review.
-
Stress-induced reversal of microRNA repression and mRNA P-body localization in human cells.Cold Spring Harb Symp Quant Biol. 2006;71:513-21. doi: 10.1101/sqb.2006.71.038. Cold Spring Harb Symp Quant Biol. 2006. PMID: 17381334 Review.
Cited by
-
MicroRNAs as effectors of brain function with roles in ischemia and injury, neuroprotection, and neurodegeneration.J Cereb Blood Flow Metab. 2010 Sep;30(9):1564-76. doi: 10.1038/jcbfm.2010.101. Epub 2010 Jul 7. J Cereb Blood Flow Metab. 2010. PMID: 20606686 Free PMC article. Review.
-
Mov10 and APOBEC3G localization to processing bodies is not required for virion incorporation and antiviral activity.J Virol. 2013 Oct;87(20):11047-62. doi: 10.1128/JVI.02070-13. Epub 2013 Aug 7. J Virol. 2013. PMID: 23926332 Free PMC article.
-
MicroRNAs as biomarkers of acute lung injury.Ann Transl Med. 2018 Jan;6(2):34. doi: 10.21037/atm.2018.01.10. Ann Transl Med. 2018. PMID: 29430451 Free PMC article. Review.
-
miR-199a, a bone morphogenic protein 2-responsive MicroRNA, regulates chondrogenesis via direct targeting to Smad1.J Biol Chem. 2009 Apr 24;284(17):11326-35. doi: 10.1074/jbc.M807709200. Epub 2009 Feb 27. J Biol Chem. 2009. PMID: 19251704 Free PMC article.
-
MicroRNAs identified in highly purified liver-derived mitochondria may play a role in apoptosis.RNA Biol. 2009 Jan-Mar;6(1):65-72. doi: 10.4161/rna.6.1.7534. Epub 2009 Jan 1. RNA Biol. 2009. PMID: 19106625 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous
