doi: 10.1016/j.ymeth.2006.07.034.
Quantitative genetic analysis in Saccharomyces cerevisiae using epistatic miniarray profiles (E-MAPs) and its application to chromatin functions
Affiliations
- PMID: 17101447
- DOI: 10.1016/j.ymeth.2006.07.034
Item in Clipboard
Quantitative genetic analysis in Saccharomyces cerevisiae using epistatic miniarray profiles (E-MAPs) and its application to chromatin functions
Methods.
2006 Dec.
Free article
Abstract
The use of the budding yeast Saccharomyces cerevisiae as a simple eukaryotic model system for the study of chromatin assembly and regulation has allowed rapid discovery of genes that influence this complex process. The functions of many of the proteins encoded by these genes have not yet been fully characterized. Here, we describe a high-throughput methodology that can be used to illuminate gene function and discuss its application to a set of genes involved in the creation, maintenance and remodeling of chromatin structure. Our technique, termed E-MAPs, involves the generation of quantitative genetic interaction maps that reveal the function and organization of cellular proteins and networks.
Similar articles
-
Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile.Cell. 2005 Nov 4;123(3):507-19. doi: 10.1016/j.cell.2005.08.031. Cell. 2005. PMID: 16269340
-
A study of biochemical and functional interactions of Htl1p, a putative component of the Saccharomyces cerevisiae, Rsc chromatin-remodeling complex.Gene. 2007 Jun 15;395(1-2):72-85. doi: 10.1016/j.gene.2007.02.002. Epub 2007 Feb 20. Gene. 2007. PMID: 17400406
-
An E-MAP of the ER.Cell. 2005 Nov 4;123(3):366-8. doi: 10.1016/j.cell.2005.10.016. Cell. 2005. PMID: 16269327
-
Exploring genetic interactions and networks with yeast.Nat Rev Genet. 2007 Jun;8(6):437-49. doi: 10.1038/nrg2085. Nat Rev Genet. 2007. PMID: 17510664 Review.
-
Noncovalent modification of chromatin: different remodeled products with different ATPase domains.Cold Spring Harb Symp Quant Biol. 2004;69:183-92. doi: 10.1101/sqb.2004.69.183. Cold Spring Harb Symp Quant Biol. 2004. PMID: 16117648 Review. No abstract available.
Cited by
-
Functional maps of protein complexes from quantitative genetic interaction data.PLoS Comput Biol. 2008 Apr 18;4(4):e1000065. doi: 10.1371/journal.pcbi.1000065. PLoS Comput Biol. 2008. PMID: 18421374 Free PMC article.
-
A UV-induced genetic network links the RSC complex to nucleotide excision repair and shows dose-dependent rewiring.Cell Rep. 2013 Dec 26;5(6):1714-24. doi: 10.1016/j.celrep.2013.11.035. Epub 2013 Dec 19. Cell Rep. 2013. PMID: 24360959 Free PMC article.
-
Chemical genetics of rapamycin-insensitive TORC2 in S. cerevisiae.Cell Rep. 2013 Dec 26;5(6):1725-36. doi: 10.1016/j.celrep.2013.11.040. Epub 2013 Dec 19. Cell Rep. 2013. PMID: 24360963 Free PMC article.
-
Key functional regions in the histone variant H2A.Z C-terminal docking domain.Mol Cell Biol. 2011 Sep;31(18):3871-84. doi: 10.1128/MCB.05182-11. Epub 2011 Jul 26. Mol Cell Biol. 2011. PMID: 21791612 Free PMC article.
-
Quantitative Yeast Genetic Interaction Profiling of Bacterial Effector Proteins Uncovers a Role for the Human Retromer in Salmonella Infection.Cell Syst. 2018 Sep 26;7(3):323-338.e6. doi: 10.1016/j.cels.2018.06.010. Epub 2018 Aug 1. Cell Syst. 2018. PMID: 30077634 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
