doi: 10.1038/nmeth979.
Epub 2006 Nov 12.
A highly sensitive protein-protein interaction assay based on Gaussia luciferase
Affiliations
- PMID: 17099704
- DOI: 10.1038/nmeth979
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A highly sensitive protein-protein interaction assay based on Gaussia luciferase
Nat Methods.
2006 Dec.
Abstract
Protein-fragment complementation assays (PCAs) provide a general strategy to study the dynamics of protein-protein interactions in vivo and in vitro. The full potential of PCA requires assays that are fully reversible and sensitive at subendogenous protein expression levels. We describe a new assay that meets these criteria, based on the Gaussia princeps luciferase enzyme, demonstrating chemical reversal, and induction and inhibition of a key interaction linking insulin and TGFbeta signaling.
Comment in
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Complementary methods for studies of protein interactions in living cells.Nat Methods. 2006 Dec;3(12):969-71. doi: 10.1038/nmeth1206-969. Nat Methods. 2006. PMID: 17117150 Free PMC article. No abstract available.
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