Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1
- PMID: 16987069
- DOI: 10.1089/vim.2006.19.518
Immunization of mice with a DNA vaccine based on severe acute respiratory syndrome coronavirus spike protein fragment 1
Erratum in
- Viral Immunol. 2006 Winter;19(4):775
Abstract
According to data in GenBank, a gene encoding SARS spike protein fragment 1 (S1) was synthesized. After recombination with an immunostimulatory sequence (ISS), the gene was cloned into the plasmid pIRES to produce pIRES-ISS-S1. On confirmation of the expression of S1 protein by indirect immunofluorescence assay (IFA), after the transfection of pIRES-ISS-S1 into BHK-21 cells, the DNA vaccine was repeatedly administrated to BALB/c mice. CD4+ and CD8+ spleen T lymphocytes were analyzed by flow cytometry (FCM) to evaluate T cell-mediated immune responses, the antigen-specific responses of T cells were evaluated by cytotoxic T lymphocyte (CTL) assay, and the level of IgG in antisera from immunized mice was determined by enzyme-linked immunosorbent assay. Results showed that the counts of spleen CD4+ and CD8+ T lymphocytes were increased, that the T cell-mediated immune responses showed antigen specificity, and that IgG was significantly induced with DNA vaccines pIRES-ISS-S1 and pIRES-S1 at titers of 1:320 and 1:160, respectively. These results are promising for the protective immunization of humans.
Similar articles
-
Priming with SARS CoV S DNA and boosting with SARS CoV S epitopes specific for CD4+ and CD8+ T cells promote cellular immune responses.Vaccine. 2007 Sep 28;25(39-40):6981-91. doi: 10.1016/j.vaccine.2007.06.047. Epub 2007 Jul 16. Vaccine. 2007. PMID: 17709158 Free PMC article.
-
Induction of specific immune responses by severe acute respiratory syndrome coronavirus spike DNA vaccine with or without interleukin-2 immunization using different vaccination routes in mice.Clin Vaccine Immunol. 2007 Jul;14(7):894-901. doi: 10.1128/CVI.00019-07. Epub 2007 May 9. Clin Vaccine Immunol. 2007. PMID: 17494640 Free PMC article.
-
SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus.Vaccine. 2005 Oct 10;23(42):4959-68. doi: 10.1016/j.vaccine.2005.05.023. Vaccine. 2005. PMID: 15993989 Free PMC article.
-
SARS Immunity and Vaccination.Cell Mol Immunol. 2004 Jun;1(3):193-8. Cell Mol Immunol. 2004. PMID: 16219167 Review.
-
Severe acute respiratory syndrome: vaccine on the way.Chin Med J (Engl). 2005 Sep 5;118(17):1468-76. Chin Med J (Engl). 2005. PMID: 16157050 Review. No abstract available.
Cited by
-
Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E. coli cells elicits potent neutralizing antibody and protective immunity.Virology. 2009 Oct 10;393(1):144-50. doi: 10.1016/j.virol.2009.07.018. Epub 2009 Aug 15. Virology. 2009. PMID: 19683779 Free PMC article.
-
Intranasal vaccination of recombinant adeno-associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and provides long-term protection against SARS-CoV infection.J Immunol. 2008 Jan 15;180(2):948-56. doi: 10.4049/jimmunol.180.2.948. J Immunol. 2008. PMID: 18178835 Free PMC article.
-
Current advancements and potential strategies in the development of MERS-CoV vaccines.Expert Rev Vaccines. 2014 Jun;13(6):761-74. doi: 10.1586/14760584.2014.912134. Epub 2014 Apr 26. Expert Rev Vaccines. 2014. PMID: 24766432 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous
