The classical srb4-138 mutant allele causes dissociation of yeast Mediator
- PMID: 16962561
- DOI: 10.1016/j.bbrc.2006.08.099
The classical srb4-138 mutant allele causes dissociation of yeast Mediator
Abstract
The Mediator complex is an essential co-activator for RNA polymerase II-dependent transcription in the budding yeast Saccharomyces cerevisiae. The S. cerevisiae core Mediator complex consists of three larger domains that are termed head, middle, and tail. The Med17 subunit is located within the head domain and is essential for cell viability. A temperature-sensitive allele of the MED17 gene known as srb4-138 causes all RNA polymerase II-dependent transcription to cease at the non-permissive temperature. The phenotype of srb4-138 allele has served as the main in vivo proof of the importance of Mediator, but the molecular basis for the effect of this mutant has not been determined. We here characterize Mediator from cells carrying the srb4-138 allele and find that the Mediator complex consistently breaks apart at the head/middle domain boundary even at lower temperatures. We find that both the head and middle domains are able to associate with the RNA polymerase independently of each other. Interestingly, both sub-complexes are able to associate with an active promoter at the permissive temperature but at the non-permissive temperature the head domain is lost from the promoter.
Similar articles
-
Head module control of mediator interactions.Mol Cell. 2006 Aug 4;23(3):355-64. doi: 10.1016/j.molcel.2006.06.007. Mol Cell. 2006. PMID: 16885025
-
Role of Gal11, a component of the RNA polymerase II mediator in stress-induced hyperphosphorylation of Msn2 in Saccharomyces cerevisiae.Mol Microbiol. 2006 Oct;62(2):438-52. doi: 10.1111/j.1365-2958.2006.05363.x. Mol Microbiol. 2006. PMID: 17020582
-
Direct interaction of RNA polymerase II and mediator required for transcription in vivo.Science. 2011 Mar 18;331(6023):1451-4. doi: 10.1126/science.1200188. Science. 2011. PMID: 21415355
-
The yeast Mediator complex and its regulation.Trends Biochem Sci. 2005 May;30(5):240-4. doi: 10.1016/j.tibs.2005.03.008. Trends Biochem Sci. 2005. PMID: 15896741 Review.
-
[Interaction between yeast transcription factor GAL11 and general transcription factors].Tanpakushitsu Kakusan Koso. 1996 Jun;41(8 Suppl):1178-86. Tanpakushitsu Kakusan Koso. 1996. PMID: 8741639 Review. Japanese. No abstract available.
Cited by
-
Architectural Mediator subunits are differentially essential for global transcription in Saccharomyces cerevisiae.Genetics. 2021 Mar 31;217(3):iyaa042. doi: 10.1093/genetics/iyaa042. Genetics. 2021. PMID: 33789343 Free PMC article.
-
Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.Cell. 2014 Jun 5;157(6):1430-1444. doi: 10.1016/j.cell.2014.05.015. Epub 2014 May 29. Cell. 2014. PMID: 24882805 Free PMC article.
-
Architecture of the RNA polymerase II-Mediator core initiation complex.Nature. 2015 Feb 19;518(7539):376-80. doi: 10.1038/nature14229. Epub 2015 Feb 4. Nature. 2015. PMID: 25652824
-
MED14 tethers mediator to the N-terminal domain of peroxisome proliferator-activated receptor gamma and is required for full transcriptional activity and adipogenesis.Mol Cell Biol. 2010 May;30(9):2155-69. doi: 10.1128/MCB.01238-09. Epub 2010 Mar 1. Mol Cell Biol. 2010. PMID: 20194623 Free PMC article.
-
Evidence that Mediator is essential for Pol II transcription, but is not a required component of the preinitiation complex in vivo.Elife. 2017 Jul 12;6:e28447. doi: 10.7554/eLife.28447. Elife. 2017. PMID: 28699889 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials
