Review
doi: 10.1385/1-59745-123-1:49.
Recognition and cleavage of primary microRNA transcripts
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- PMID: 16957366
- DOI: 10.1385/1-59745-123-1:49
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Review
Recognition and cleavage of primary microRNA transcripts
Methods Mol Biol.
2006.
Abstract
MicroRNAs (miRNAs) are approx 22-nucleotide (nt)-long, single-stranded, endogenous, noncoding RNAs that are widely expressed in multicellular organisms. This chapter describes methods that allow the overexpression of human miRNAs and also discusses how primary miRNAs (pri-miRNAs), the much longer precursors of mature miRNAs, are processed in human cells, as well as in vitro.
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