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. 2006 Sep 29;313(5795):1968-72.
doi: 10.1126/science.1131981. Epub 2006 Aug 24.

Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

Christian B F Andersen  1 , Lionel Ballut, Jesper S Johansen, Hala Chamieh, Klaus H Nielsen, Cristiano L P Oliveira, Jan Skov Pedersen, Bertrand Séraphin, Hervé Le Hir, Gregers Rom Andersen
Affiliations

Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA

Christian B F Andersen et al. Science. .

Abstract

In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

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