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Comparative Study
. 1990 Mar 29;344(6265):435-9.
doi: 10.1038/344435a0.

New type of POU domain in germ line-specific protein Oct-4

H R Schöler  1 , S Ruppert, N Suzuki, K Chowdhury, P Gruss
Affiliations
Comparative Study

New type of POU domain in germ line-specific protein Oct-4

H R Schöler et al. Nature. .

Abstract

Members of a family of murine octamer-binding proteins interact specifically with the octamer motif, a transcription regulatory element found in the promoter and enhancer regions of many genes. Oct-4 is a maternally expressed protein that is also present in the pre-implantation mouse embryo. Although many regulatory proteins are expressed in post-implantation embryos, transcription factors regulating pre-implantation processes have remained elusive. The Oct-4 gene is therefore a prime candidate for an early developmental control gene. Here we report the complementary DNA cloning of the mouse Oct-4 gene, and the characterization of the encoded protein(s) by sequential in vitro transcription, translation, DNA-binding and protease-clipping analysis. Deletion analysis shows that the DNA-binding activity is mediated by a POU domain encoded in an open reading frame corresponding to a 324-amino-acid protein. Sequence comparison with known POU domains reveals that Oct-4 is a novel member of the POU-family.

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