Control of Listeria superoxide dismutase by phosphorylation
- PMID: 16905535
- DOI: 10.1074/jbc.M606249200
Control of Listeria superoxide dismutase by phosphorylation
Abstract
Superoxide dismutases (SODs) are enzymes that protect organisms against superoxides and reactive oxygen species (ROS) produced during their active metabolism. ROS are major mediators of phagocytes microbicidal activity. Here we show that the cytoplasmic Listeria monocytogenes MnSOD is phosphorylated on serine and threonine residues and less active when bacteria reach the stationary phase. We also provide evidence that the most active nonphosphorylated form of MnSOD can be secreted via the SecA2 pathway in culture supernatants and in infected cells, where it becomes phosphorylated. A Deltasod deletion mutant is impaired in survival within macrophages and is dramatically attenuated in mice. Together, our results demonstrate that the capacity to counteract ROS is an essential component of L. monocytogenes virulence. This is the first example of a bacterial SOD post-translationally controlled by phosphorylation, suggesting a possible new host innate mechanism to counteract a virulence factor.
Similar articles
-
Expression of superoxide dismutase in Listeria monocytogenes.Appl Environ Microbiol. 1994 Jul;60(7):2360-6. doi: 10.1128/aem.60.7.2360-2366.1994. Appl Environ Microbiol. 1994. PMID: 8074517 Free PMC article.
-
Inactivation of the sodA gene of Streptococcus suis type 2 encoding superoxide dismutase leads to reduced virulence to mice.Vet Microbiol. 2012 Aug 17;158(3-4):360-6. doi: 10.1016/j.vetmic.2012.02.028. Epub 2012 Feb 28. Vet Microbiol. 2012. PMID: 22424868
-
Interaction with enzyme IIBMpo (EIIBMpo) and phosphorylation by phosphorylated EIIBMpo exert antagonistic effects on the transcriptional activator ManR of Listeria monocytogenes.J Bacteriol. 2015 May;197(9):1559-72. doi: 10.1128/JB.02522-14. Epub 2015 Feb 17. J Bacteriol. 2015. PMID: 25691525 Free PMC article.
-
Managing odds in stem cells: insights into the role of mitochondrial antioxidant enzyme MnSOD.Free Radic Res. 2016;50(5):570-84. doi: 10.3109/10715762.2016.1155708. Free Radic Res. 2016. PMID: 26899340 Review.
-
Chemical Warfare at the Microorganismal Level: A Closer Look at the Superoxide Dismutase Enzymes of Pathogens.ACS Infect Dis. 2018 Jun 8;4(6):893-903. doi: 10.1021/acsinfecdis.8b00026. Epub 2018 Mar 14. ACS Infect Dis. 2018. PMID: 29517910 Free PMC article. Review.
Cited by
-
Illuminating the landscape of host-pathogen interactions with the bacterium Listeria monocytogenes.Proc Natl Acad Sci U S A. 2011 Dec 6;108(49):19484-91. doi: 10.1073/pnas.1112371108. Epub 2011 Nov 23. Proc Natl Acad Sci U S A. 2011. PMID: 22114192 Free PMC article. Review.
-
The ins and outs of Mycobacterium tuberculosis protein export.Tuberculosis (Edinb). 2012 Mar;92(2):121-32. doi: 10.1016/j.tube.2011.11.005. Epub 2011 Dec 21. Tuberculosis (Edinb). 2012. PMID: 22192870 Free PMC article. Review.
-
Rhombencephalitis Caused by Listeria monocytogenes in Humans and Ruminants: A Zoonosis on the Rise?Interdiscip Perspect Infect Dis. 2010;2010:632513. doi: 10.1155/2010/632513. Epub 2010 Feb 28. Interdiscip Perspect Infect Dis. 2010. PMID: 20204066 Free PMC article.
-
Listeria monocytogenes PrsA2 is required for virulence factor secretion and bacterial viability within the host cell cytosol.Infect Immun. 2010 Nov;78(11):4944-57. doi: 10.1128/IAI.00532-10. Epub 2010 Sep 7. Infect Immun. 2010. PMID: 20823208 Free PMC article.
-
Acid shock of Listeria monocytogenes at low environmental temperatures induces prfA, epithelial cell invasion, and lethality towards Caenorhabditis elegans.BMC Genomics. 2013 Apr 27;14:285. doi: 10.1186/1471-2164-14-285. BMC Genomics. 2013. PMID: 23622257 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
