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. 2006 Aug;44(8):2974-6.
doi: 10.1128/JCM.01021-06.

Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species

Jane F Turton  1 , Neil Woodford, Judith Glover, Susannah Yarde, Mary E Kaufmann, Tyrone L Pitt
Affiliations

Identification of Acinetobacter baumannii by detection of the blaOXA-51-like carbapenemase gene intrinsic to this species

Jane F Turton et al. J Clin Microbiol. 2006 Aug.

Abstract

bla(OXA-51-like) was sought in clinical isolates of Acinetobacter species in a multiplex PCR, which also detects bla(OXA-23-like) and class 1 integrase genes. All isolates that gave a band for bla(OXA-51-like) identified as A. baumannii. This gene was detected in each of 141 isolates of A. baumannii but not in those of 22 other Acinetobacter species.

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Figures

FIG. 1.
FIG. 1.
Multiplex PCR results to amplify fragments of blaOXA-23-like, blaOXA-51-like, and the class 1 integrase gene from Acinetobacter spp. Representatives of OXA-23 clone 1 (lanes 1, 4, 5, 7, 8, 9, 11, and 12), the SE clone (lanes 16 and 17), and strains A (lane 6), C (lanes 2 and 3), and D (lane 10) are included. Isolates in lanes 13, 14, and 15 were sporadic. All isolates except that in lane 13 identified as A. baumannii and had blaOXA-51-like; the isolate in lane 13 was identified as A. lwoffii and lacked this gene. M, 123-bp ladder.
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