Comparison of multiple shell vial cell lines for isolation of enteroviruses: a national perspective
- PMID: 16884950
- DOI: 10.1016/j.jcv.2006.06.009
Comparison of multiple shell vial cell lines for isolation of enteroviruses: a national perspective
Abstract
Background: Isolation of enterovirus in cell culture is still utilized by clinical laboratories, for vaccine research, and for identifying serotypes for disease surveillance. Use of a combination of cell lines is recommended yet this practice has not been rigorously examined for shell vials.
Objectives: To evaluate the growth predilection of enterovirus serotypes for certain shell vial cell lines in clinical samples received at a national reference laboratory.
Study design: We retrospectively reviewed results of samples submitted for viral culture over a 3-year period. PMK, BGM, RD, A549, and MRC-5 cell lines grown in shell vials were inoculated and serotyped.
Results: Of 55,816 cultures, 1047 (1.9%) yielded enterovirus representing 18 serotypes. Echovirus 7, echovirus 9, and echovirus 30 were the most common serotypes recovered in 2002, 2003, and 2004, respectively. PMK and MRC-5 recovered the majority of enterovirus isolates; the addition of BGM and RD cells increased our recovery rate by 13%. For 52.6% of enteroviral isolates, cytopathic effect was found in only a single cell line. PMK and BGM cells were effective in isolating coxsackieviruses, and RD and MRC-5 were useful particularly in isolating echoviruses.
Conclusions: A combination of shell vial cell lines is still recommended for recovery of enteroviruses.
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