Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 Sep;54(4):316-28.
doi: 10.1002/glia.20384.

Regulation of the astrocyte resting membrane potential by cyclic AMP and protein kinase A

Sally Bolton  1 , Kirsty Greenwood, Nicola Hamilton, Arthur M Butt
Affiliations

Regulation of the astrocyte resting membrane potential by cyclic AMP and protein kinase A

Sally Bolton et al. Glia. 2006 Sep.

Abstract

The factors regulating astroglial resting membrane potential (RMP) are unresolved. Here, we have examined developmental, morphological, and intracellular factors that may regulate the RMP in astrocytes of isolated intact optic nerves of rats and mice aged postnatal day (P3) to adult. The astroglial RMP ranged from -25 to -85 mV, independent of age and morphological phenotype. There was a developmental negative shift in the astroglial RMP from a non-Gaussian distribution in perinatal nerves, to a bimodal distribution of RMPs after P15, with peaks at -52 and -74 mV in adults. Blockade of Kir with 100 microM BaCl(2) significantly depolarized the RMP to -30 mV; the RMP was unaffected by TEA or agents that modulated ATP-sensitive potassium channels. Raising intracellular cyclic AMP (cAMP) with dbcAMP or forskolin induced a significant hyperpolarization by approximately 15 mV, whereas inhibition of cAMP-dependent protein kinase (PKA) depolarized the astroglial RMP to -40 mV. The hyperpolarizing action of dbcAMP was blocked by 100 microM BaCl(2). The effects of BaCl(2) indicate that the developmental negative shift in the RMP and the cAMP-mediated hyperpolarization were dependent on Kir. This study provides evidence that the heterogeneous RMP of mature astrocytes is regulated by cAMP and PKA signaling.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

Publication types

MeSH terms

LinkOut - more resources