Continuous dielectrophoretic size-based particle sorting
- PMID: 16841925
- DOI: 10.1021/ac0601314
Continuous dielectrophoretic size-based particle sorting
Abstract
Continuous-flow dielectrophoretic (DEP) particle separation based on size is demonstrated in a microfluidic device. Polystyrene microspheres suspended in a neutrally buoyant aqueous solution are used as model particles to study DEP induced by an array of slanted, planar, interdigitated electrodes inside of a soft-lithography microchannel. The E-field gradients from the slanted electrodes impart a net transverse force component on the particles that causes them to "ratchet" across the channel. Over the length of the device, larger particles are deflected more than smaller particles according to the balance of hydrodynamic drag and DEP forces. Consequently, a flow-focused particle suspension containing different-sized particles is fractionated as the beads flow and separate down the length of the device. The flow behavior of spherical particles is modeled, and the total transverse particle displacement in the microfluidic device predicts fourth-order size and voltage and second-order inverse flow rate dependences. The model is verified experimentally for a range of flow rates, particle sizes, and E-field strengths.
Similar articles
-
Lateral displacement as a function of particle size using a piecewise curved planar interdigitated electrode array.Lab Chip. 2009 Oct 21;9(20):2958-64. doi: 10.1039/b909753h. Epub 2009 Aug 5. Lab Chip. 2009. PMID: 19789750
-
Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow-through separation of beads and cells.Electrophoresis. 2009 Mar;30(5):782-91. doi: 10.1002/elps.200800637. Electrophoresis. 2009. PMID: 19197906
-
Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array.Lab Chip. 2005 Oct;5(10):1161-7. doi: 10.1039/b505088j. Epub 2005 Aug 2. Lab Chip. 2005. PMID: 16175274
-
Dielectrophoretic separation of bioparticles in microdevices: a review.Electrophoresis. 2014 Mar;35(5):691-713. doi: 10.1002/elps.201300424. Epub 2014 Feb 4. Electrophoresis. 2014. PMID: 24338825 Review.
-
Handling and analysis of cells and bioparticles on centrifugal microfluidic platforms.Expert Rev Mol Diagn. 2012 May;12(4):407-21. doi: 10.1586/erm.12.28. Expert Rev Mol Diagn. 2012. PMID: 22616705 Review.
Cited by
-
Multitarget dielectrophoresis activated cell sorter.Anal Chem. 2008 Nov 15;80(22):8656-61. doi: 10.1021/ac8015938. Epub 2008 Oct 22. Anal Chem. 2008. PMID: 18939853 Free PMC article.
-
Vertical Hydrodynamic Focusing and Continuous Acoustofluidic Separation of Particles via Upward Migration.Adv Sci (Weinh). 2017 Dec 22;5(2):1700285. doi: 10.1002/advs.201700285. eCollection 2018 Feb. Adv Sci (Weinh). 2017. PMID: 29619294 Free PMC article.
-
Vector separation of particles and cells using an array of slanted open cavities.Lab Chip. 2013 Mar 21;13(6):1086-92. doi: 10.1039/c2lc40927e. Lab Chip. 2013. PMID: 23306214 Free PMC article.
-
Dielectrophoretic sorting of membrane protein nanocrystals.ACS Nano. 2013 Oct 22;7(10):9129-37. doi: 10.1021/nn403760q. Epub 2013 Sep 9. ACS Nano. 2013. PMID: 24004002 Free PMC article.
-
Dielectrophoresis for bioparticle manipulation.Int J Mol Sci. 2014 Oct 10;15(10):18281-309. doi: 10.3390/ijms151018281. Int J Mol Sci. 2014. PMID: 25310652 Free PMC article. Review.
LinkOut - more resources
Full Text Sources
Other Literature Sources
