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. 2006 May;1759(5):195-207.
doi: 10.1016/j.bbaexp.2006.04.010. Epub 2006 May 22.

Molecular properties and intracellular localization of rat liver nuclear scaffold protein P130

Yasuhide Hibino  1 , Tatsuhiro Usui, Yasuhiro Morita, Noriko Hirose, Mari Okazaki, Nobuhiko Sugano, Koichi Hiraga
Affiliations

Molecular properties and intracellular localization of rat liver nuclear scaffold protein P130

Yasuhide Hibino et al. Biochim Biophys Acta. 2006 May.

Abstract

We examined the molecular basis of rat P130, a nuclear scaffold protein, and its functions. P130 comprising 845 amino acid residues possesses several functional domains and yields an electrophoretically distinctive isoform, P123, by altering its phosphorylation status in association with translocation across the nuclear membrane and from the digitonin-extractable fraction of the nucleus to the nuclear scaffold. The functional domains, NLS, NES, and zinc-finger bearing DNA-binding domains, ZF1 and ZF2, aid these translocations. P130 binds RNA through two RNA-binding domains (RB1 and RB2) similar to those of hnRNPs I and L. Microsome- and polysome-localized P130 and P123 were found in rat liver and Ac2F hepatoma cells. This localization required prior entry of P130 to the nucleus, but did not require RB1 and RB2. Thus, P130 initially purified from rat liver nuclear scaffold has the potential to play a variety of roles in biological events not only in the nuclear scaffold but also in various subcellular compartments. P130 (AB205483) is identical to matrin 3 (M63485 and BC062231), although the primary structure of rat matrin 3 has been revised, since it was first published.

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