Human neuroblastoma in nude mice
- PMID: 167965
Human neuroblastoma in nude mice
Abstract
For establishment of a reproducible model of human neuroblastoma, 2 to 5 million of established neuroblastoma cell lines (SK-N-SH, SK-N-MC) were injected s.c. or i.p. into 20 nu/nu mice of a predominantly Swiss back-ground. Following latency periods of 8 to 21 days, tumors developed at the injection site and grew to 4-ml volumes within 3 weeks. Histologically, the tumors resembled the original metastases from which the tumors were derived; however, the SK-N-SH appeared to have evidence of morphological differentiation. When compared to monolayer culture, the heterotransplanted SK-N-SH tumor had decreased dopamine-beta-hydroxylase activity and elevated cyclic adenosine 3':5'-monophosphate phosphodiesterase activity. Activity of cyclic adenosine 3':5'-monophosphate phosphodiesterase in the transplanted SK-N-MC tumor was not appreciably different from the activity in the cultured cells. Serum dopamine-beta-hydroxylase levels in the mice bearing SK-N-SH tumor increased threefold. The SK-N-MC cultured cells lacked dopamine-beta-hydroxylase and did not alter existing serum levels in the SK-N-MC tumor-bearing mice. 67Ga injected i.v. was found to localize in the tumor after 24 hr. Human neuroblastoma in the nude mouse can be a reproducible and informative model for tumor pharmacology, screening, radionuclides, tumor localization and imaging, and investigating morphological differentiation.
Similar articles
-
Integrin expression in human neuroblastoma cells with or without N-myc amplification and in ectopic/orthotopic nude mouse tumors.Exp Cell Res. 1994 Jul;213(1):156-63. doi: 10.1006/excr.1994.1185. Exp Cell Res. 1994. PMID: 8020586
-
Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.Cancer Res. 1977 May;37(5):1364-71. Cancer Res. 1977. PMID: 856461
-
In vitro and in vivo preclinical chemotherapy studies of human neuroblastoma.Cancer Treat Rep. 1980 Aug-Sep;64(8-9):975-9. Cancer Treat Rep. 1980. PMID: 7448832
-
Metastases of human tumors in experimental animals.Anticancer Res. 1987 Sep-Oct;7(5B):997-1003. Anticancer Res. 1987. PMID: 3324940 Review.
-
Considerations in the use of nude mice for cancer research.Cancer Metastasis Rev. 1984;3(4):341-60. doi: 10.1007/BF00051459. Cancer Metastasis Rev. 1984. PMID: 6394126 Review.
Cited by
-
Growth of human colonic adenocarcinoma and development of serum CEA in in athymic mice. I: Strict correlation of tumour size and mass with serum CEA concentration during logarithmic growth.Br J Cancer. 1982 Dec;46(6):841-7. doi: 10.1038/bjc.1982.293. Br J Cancer. 1982. PMID: 7150482 Free PMC article.
-
Anti-cancer effect of HIV-1 viral protein R on doxorubicin resistant neuroblastoma.PLoS One. 2010 Jul 7;5(7):e11466. doi: 10.1371/journal.pone.0011466. PLoS One. 2010. PMID: 20628645 Free PMC article.
-
In vitro and in vivo inhibition of SK-N-MC neuroblastoma growth using cyclic nucleotide phosphodiesterase inhibitors.J Neurooncol. 2001 Jan;51(1):25-31. doi: 10.1023/a:1006489020190. J Neurooncol. 2001. PMID: 11349877
-
Genipin promotes the apoptosis and autophagy of neuroblastoma cells by suppressing the PI3K/AKT/mTOR pathway.Sci Rep. 2024 Aug 30;14(1):20231. doi: 10.1038/s41598-024-71123-w. Sci Rep. 2024. PMID: 39215133 Free PMC article.
-
Magnetic resonance imaging for the evaluation of a novel metastatic orthotopic model of human neuroblastoma in immunodeficient mice.Clin Exp Metastasis. 2000;18(6):455-61. doi: 10.1023/a:1011827122126. Clin Exp Metastasis. 2000. PMID: 11592302
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials